Tissue pool of hypersegmented neutrophils in patients with precancerous and tumor diseases of the larynx and laryngopharynx
- Authors: Kologrivova E.N.1, Pleshko R.I.1, Cheremisina O.V.2, Boldyshevskaya M.A.2, Gereng E.A.1, Nasibov T.F.1
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Affiliations:
- Siberian State Medical University
- Тomsk National Research Medical Center, Russian Academy of Sciences
- Issue: Vol 27, No 4 (2024)
- Pages: 929-938
- Section: SHORT COMMUNICATIONS
- URL: https://journals.rcsi.science/1028-7221/article/view/267909
- DOI: https://doi.org/10.46235/1028-7221-16626-TPO
- ID: 267909
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Abstract
The presence of neutrophils in a tumor often correlates with an unfavorable prognosis, however, there is still no clear answer about the role of tumor-associated neutrophils and the relationship of their morphofunctional features with the prognosis of the course of the disease. The aim of the study was to evaluate the structural features of tissue neutrophil nuclei in the pathological zone in patients with precancerous changes and malignant neoplasms in the larynx. Eight people with precancerous changes in the larynx, 18 patients with localized cancer of the larynx (stages II and III of the tumor process and the absence of metastases – T2- 3N0M0), and 12 patients with advanced cancer (stage III of tumor diseases and regional metastases – T3N1- 2M0) were examined. Blood slides and smear prints of tissue biopsies from three localizations were examined: 1 – zones of the pathological focus; 2 – boundaries between the pathological focus and conditionally healthy tissue; and 3 – conditionally healthy tissue. In the blood of patients with precancerous changes in the larynx and cancer patients, the main part of neutrophils was represented by cells with a 4-segment nucleus, and the number of hypersegmented forms (5 or more segments) was higher than in the blood of healthy volunteers (p < 0.002). The same type of changes in smear prints of biopsies from different areas of the pathological focus in patients with precancerous and malignant neoplasms were revealed: as they approached the pathological focus, an increase the cells with 4-5 or more segments was observed. The content of hypersegmented forms was maximum in the pathological focus. Only in patients with a widespread tumor process, neutrophils with a 4-segment nucleus dominated the immediate environment of the tumor. As the tumor spreads and metastases form, the proportion of hypersegmented cells increases in the population of intra-tumor neutrophils. It is very likely that the morphological features of tissue neutrophils in different zones of the pathological focus reflect their functional heterogeneity, and hypersegmetation of nuclei can be considered as a potential predictor of the development and progression of the tumor process.
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##article.viewOnOriginalSite##About the authors
E. N. Kologrivova
Siberian State Medical University
Author for correspondence.
Email: enkologrivova@mail.ru
PhD, MD (Medicine), Professor, Department of Immunology and Allergology
Russian Federation, 2 Moskovsky Trakt, Тomsk, 643050R. I. Pleshko
Siberian State Medical University
Email: enkologrivova@mail.ru
PhD, MD (Medicine), Professor, Department of Morphology and General Pathology
Russian Federation, 2 Moskovsky Trakt, Тomsk, 643050O. V. Cheremisina
Тomsk National Research Medical Center, Russian Academy of Sciences
Email: enkologrivova@mail.ru
PhD, MD (Medicine), Head, Endoscopy Department, Cancer Research Institute
Russian Federation, ТomskM. A. Boldyshevskaya
Тomsk National Research Medical Center, Russian Academy of Sciences
Email: enkologrivova@mail.ru
Doctor of Clinical Laboratory Diagnostics, Pathological and Anatomical Department, Cancer Research Institute
Russian Federation, ТomskE. A. Gereng
Siberian State Medical University
Email: enkologrivova@mail.ru
PhD, MD (Medicine), Professor, Department of Morphology and General Pathology
Russian Federation, 2 Moskovsky Trakt, Тomsk, 643050T. F. Nasibov
Siberian State Medical University
Email: enkologrivova@mail.ru
5th year Sudent
Russian Federation, 2 Moskovsky Trakt, Тomsk, 643050References
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