Multilayer film of thiourea and gold nanoparticles as an effective platform for immobilization of activated non-labeled-DNA and construction of an ultrasensitive electrochemical DNA biosensor
- 作者: Mashhadizadeh M.1, Talemi R.1
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隶属关系:
- Faculty of Chemistry
- 期: 卷 52, 编号 2 (2016)
- 页面: 154-162
- 栏目: Article
- URL: https://journals.rcsi.science/1023-1935/article/view/187667
- DOI: https://doi.org/10.1134/S1023193515080078
- ID: 187667
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详细
In this work, self assembly of thiourea and gold nano-particle multilayer built up on a thiourea modified gold nanoparticles Au electrode, has been used as a platform for immobilization of activated ss-DNA. Two NH2 group of thiourea on a multilayer surface can interact with an activated phosphate group of non-labeled ss-DNA. Activated non-labeled ss-DNA was prepared using N-(3 dimethylaminopropyl)-N-ethyl-carbodiimide hydrochloride (EDC) and N-hydroxy-succinimide (NHS). The whole DNA biosensor fabrication process was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods with the use of ferricyanide as an electrochemical redox indicator. Methylene Blue (MB) was used as the electrochemical indicator for monitoring the hybridization reaction after hybridized with the target ssDNA and the reduction current of MB intercalation decreased with increasing the concentration of target DNA, ranging from 7.9 × 10–13 to 1.2 × 10–8 M with a very low detection limit of 3.8 × 10–13 M (S/N = 3).
作者简介
Mohammad Mashhadizadeh
Faculty of Chemistry
编辑信件的主要联系方式.
Email: mashhadizadeh@khu.ac.ir
伊朗伊斯兰共和国, Tehran
Rasoul Talemi
Faculty of Chemistry
Email: mashhadizadeh@khu.ac.ir
伊朗伊斯兰共和国, Tehran