Inheritance of IncP-9 Catabolic Plasmids in Pseudomonas Bacteria
- Autores: Esikova T.Z.1, Anokhina T.O.1, Akhmetov L.I.1, Kosheleva I.A.1, Boronin A.M.1
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Afiliações:
- Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences
- Edição: Volume 55, Nº 12 (2019)
- Páginas: 1569-1572
- Seção: Short Communications
- URL: https://journals.rcsi.science/1022-7954/article/view/189892
- DOI: https://doi.org/10.1134/S1022795419120032
- ID: 189892
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Resumo
This work investigated the capability of the conjugative transfer of epsilon-caprolactam biodegradation (CAP) plasmids, belonging to incompatibility (Inc) groups P-2, P-7, P-9, and IncP-9 naphthalene biodegradation (NAH) plasmids to various species of Pseudomonas bacteria, including strains of plant growth-promoting rhizobacteria (PGPR), and the character of plasmid inheritance in these strains. The frequency of the conjugative transfer of all CAP and NAH plasmids (irrespective of their Inc groups) into Pseudomonas PGPR was shown to be lower than that into P. putida soil strains by more than an order of magnitude. The low frequency of conjugative transfer was found not to be caused by secretion of antibiotically active metabolites by Pseudomonas PGPR strains. Stability of inheritance of catabolic plasmids under nonselective conditions was established to depend on their Inc group and bacterial host strain. Thus, all investigated plasmids were stably maintained in P. putida KT2442 and BS394 strains for at least 100 generations. However, only CAP plasmids of IncP-2 and IncP-7 groups were stably maintained in Pseudomonas PGPR cells. CAP and NAH plasmids of different IncP-9 subgroups were eliminated from cells of rhizosphere pseudomonads within several generations. Thus, the work first revealed inheritance features of IncP-9 catabolic plasmids in Pseudomonas PGPR cells.
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Sobre autores
T. Esikova
Skryabin Institute of Biochemistry and Physiology of Microorganisms,Russian Academy of Sciences
Autor responsável pela correspondência
Email: das3534@rambler.ru
Rússia, Pushchino, Moscow oblast, 142290
T. Anokhina
Skryabin Institute of Biochemistry and Physiology of Microorganisms,Russian Academy of Sciences
Email: das3534@rambler.ru
Rússia, Pushchino, Moscow oblast, 142290
L. Akhmetov
Skryabin Institute of Biochemistry and Physiology of Microorganisms,Russian Academy of Sciences
Email: das3534@rambler.ru
Rússia, Pushchino, Moscow oblast, 142290
I. Kosheleva
Skryabin Institute of Biochemistry and Physiology of Microorganisms,Russian Academy of Sciences
Email: das3534@rambler.ru
Rússia, Pushchino, Moscow oblast, 142290
A. Boronin
Skryabin Institute of Biochemistry and Physiology of Microorganisms,Russian Academy of Sciences
Email: das3534@rambler.ru
Rússia, Pushchino, Moscow oblast, 142290
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