Enviar artigo por via de e-mail Development of microsatellite genetic markers in Siberian stone pine (Pinus sibirica Du Tour) based on the de novo whole genome sequencing
- Autores: Belokon M.M.1, Politov D.V.1, Mudrik E.A.1, Polyakova T.A.1,2, Shatokhina A.V.1, Belokon Y.S.1, Oreshkova N.V.3,4, Putintseva Y.A.3,4, Sharov V.V.4, Kuzmin D.A.4, Krutovsky K.V.1,4,5,6
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Afiliações:
- Vavilov Institute of General Genetics
- Russian Center of Forest Health
- Sukachev Institute of Forest
- Genome Research and Education Center
- Georg-August University of Göttingen
- Texas A&M University
- Edição: Volume 52, Nº 12 (2016)
- Páginas: 1263-1271
- Seção: Plant Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/188027
- DOI: https://doi.org/10.1134/S1022795416120036
- ID: 188027
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Resumo
Siberian stone pine, Pinus sibirica Du Tour is one of the most economically and environmentally important forest-forming species of conifers in Russia. To study these forests a large number of highly polymorphic molecular genetic markers, such as microsatellite loci, are required. Prior to the new high-throughput next generation sequencing (NGS) methods, discovery of microsatellite loci and development of micro-satellite markers were very time consuming and laborious. The recently developed draft assembly of the Siberian stone pine genome, sequenced using the NGS methods, allowed us to identify a large number of microsatellite loci in the Siberian stone pine genome and to develop species-specific PCR primers for amplification and genotyping of 70 microsatellite loci. The primers were designed using contigs containing short simple sequence tandem repeats from the Siberian stone pine whole genome draft assembly. Based on the testing of primers for 70 microsatellite loci with tri-, tetra- or pentanucleotide repeats, 18 most promising, reliable and polymorphic loci were selected that can be used further as molecular genetic markers in population genetic studies of Siberian stone pine.
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Sobre autores
M. Belokon
Vavilov Institute of General Genetics
Email: kkrutovsky@gmail.com
Rússia, Moscow, 119991
D. Politov
Vavilov Institute of General Genetics
Email: kkrutovsky@gmail.com
Rússia, Moscow, 119991
E. Mudrik
Vavilov Institute of General Genetics
Email: kkrutovsky@gmail.com
Rússia, Moscow, 119991
T. Polyakova
Vavilov Institute of General Genetics; Russian Center of Forest Health
Email: kkrutovsky@gmail.com
Rússia, Moscow, 119991; Pushkino, 141207
A. Shatokhina
Vavilov Institute of General Genetics
Email: kkrutovsky@gmail.com
Rússia, Moscow, 119991
Yu. Belokon
Vavilov Institute of General Genetics
Email: kkrutovsky@gmail.com
Rússia, Moscow, 119991
N. Oreshkova
Sukachev Institute of Forest; Genome Research and Education Center
Email: kkrutovsky@gmail.com
Rússia, Krasnoyarsk, 660036; Krasnoyarsk, 660036
Yu. Putintseva
Sukachev Institute of Forest; Genome Research and Education Center
Email: kkrutovsky@gmail.com
Rússia, Krasnoyarsk, 660036; Krasnoyarsk, 660036
V. Sharov
Genome Research and Education Center
Email: kkrutovsky@gmail.com
Rússia, Krasnoyarsk, 660036
D. Kuzmin
Genome Research and Education Center
Email: kkrutovsky@gmail.com
Rússia, Krasnoyarsk, 660036
K. Krutovsky
Vavilov Institute of General Genetics; Genome Research and Education Center; Georg-August University of Göttingen; Texas A&M University
Autor responsável pela correspondência
Email: kkrutovsky@gmail.com
Rússia, Moscow, 119991; Krasnoyarsk, 660036; Göttingen, D-37077; College Station, TX, 77843-2138
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