Analysis of Spring Triticale Collection for Leaf Rust Resistance Genes with PCR Markers

Resumo

The results of PCR analysis of the collection of spring triticale accessions for the presence of genes Lr9, Lr12, Lr19, Lr24, Lr25, Lr28, Lr29, and Lr47 (conferring resistance to wheat leaf (brown) rust caused by Puccinia triticina Erikss.) with the use of molecular markers and isogenic lines carrying target genes (as a positive control) are presented in this article. The absence of positive PCR amplification of the DNA markers for the Lr9, Lr24, Lr28, Lr29, and Lr47 genes is observed in all the studied accessions of the spring triticale collection. The accessions Lena 1270, 25AD20, k-1763, k-3256, and Arta 59 are found to carry the Xgwm251 marker allele of the same size as that of isogenic Thatcher line with Lr25. PCR analysis using the LrAg marker shows that such triticale accessions as Pamyati Merezhko, Ulyana, V20-140, S17, PRAG 554/1, C95, 08871, RIL-130 R22-2, 172-1-16, C250, 08857, 09228, 131/17, A2-16-11, POPW9, PRAG 500, C260, Arta116/2, PRAG 554, AVS19883, k-1220, PRAG 553/1, C254, PRAG 518, PRAG 418, R-7-5 RIL202, L2413, and L8-6 carry a fragment close in size to that of the isogenic Thatcher line with Lr19 (used as positive control). Thus, we have shown that the gene pool of spring triticale is extremely depleted in leaf rust resistance genes. Active work is required on the introgression of new resistance genes both from the known donor lines of triticale and from bread wheat.