Relationship of Bovine Lymphocyte Antigen Genes with Clinical Mastitis Disease Using SSCP Technique
- Autores: Firouzamandi M.1, Eshghi D.2, Toloui M.3, Asadpour R.3
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Afiliações:
- Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz
- Veterinary Reproductive and Obstetrics, University of Shiraz
- Department of Clinical Science, University of Tabriz
- Edição: Volume 55, Nº 4 (2019)
- Páginas: 473-479
- Seção: Animal Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/189327
- DOI: https://doi.org/10.1134/S1022795419040033
- ID: 189327
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Resumo
BoLA-A and BoLA-DRB3 are in class I and II genes of bovine lymphocyte antigen of immune system respectively which cattle express per haplotype. The aim of this study was to evaluate relationship of BoLA-A and BoLA-DRB3 polymorphisms with clinical mastitis disease using SSCP technique. In the current study, blood sampling was carried out from Holstein cattle (n = 50 susceptible and n = 50 resistant to clinical mastitis) and a random sampling was conducted from Iranian native cattle (Sarabi; n = 50). Amplification of exon 2 of BoLA-DRB3 and BoLA-A genes by specific primers was used for SSCP technique. SSCP technique successfully able to detect some pattern is related to clinical mastitis disease in BoLA-DRB3.2 region as well as genotype pattern of C was observed significantly (P < 0.05) in susceptible Holstein cattle in compared to others pattern. Also, B and Q genotypes patterns of SSCP were detected only in susceptible cattle. Also, SSCP results were showed 17 genotypes pattern in Holstein and 17 different genotypes pattern in Sarabi cattle in BoLA-DRB3.2 gene. Thus, our finding indicated that Sarabi breed comprises completely different allelic in the BoLA-DRB3.2 region compared to Holstein breed. Moreover, results of this study revealed that exon 2 of BoLA-A showed lack of polymorphism in both studied breed. This is the first study on characterization of exon 2 of BoLA-Agene using SSCP technique in bovine.
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Sobre autores
M. Firouzamandi
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz
Autor responsável pela correspondência
Email: m.firouzamandi@tabrizu.ac.ir
Irã, Tabriz, 5166616471
D. Eshghi
Veterinary Reproductive and Obstetrics, University of Shiraz
Email: m.firouzamandi@tabrizu.ac.ir
Irã, Shiraz
M. Toloui
Department of Clinical Science, University of Tabriz
Email: m.firouzamandi@tabrizu.ac.ir
Irã, Tabriz, 5166616471
R. Asadpour
Department of Clinical Science, University of Tabriz
Email: m.firouzamandi@tabrizu.ac.ir
Irã, Tabriz, 5166616471