Мақаланы E-mail арқылы жіберу Molecular characterization and expression analysis of pathogenesis related protein 6 from Panax ginseng
- Авторлар: Myagmarjav D.1, Sukweenadhi J.1, Kim Y.J.2, Jang M.G.2, Rahimi S.1, Silva J.1, Choi J.Y.2, Mohanan P.1, Kwon W.S.2, Kim C.G.2, Yang D.1,2
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Мекемелер:
- Graduate School of Biotechnology and Ginseng Bank, College of Life Science
- Department of Oriental Medicinal Biotechnology, College of Life Science
- Шығарылым: Том 53, № 11 (2017)
- Беттер: 1211-1220
- Бөлім: Plant Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/188515
- DOI: https://doi.org/10.1134/S1022795417110060
- ID: 188515
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Аннотация
Panax ginseng Meyer is one of the important medicinal plants in the world, particularly in Asian countries. Ginseng encounters many stress exposure during its long cultivation period. However, the molecular mechanism of stress resistance is still poorly understood in spite of its importance. In this study, pathogenesis-related protein 6 (PR6), also called proteinase inhibitor (PI), was isolated from ginseng embryogenic callus, named PgPR6. The small size of PR6, containing an open reading frame of 219 bp encoding 72 amino acids, the typical characteristic of PR6 protein, shares the highest sequence similarity to PR6 of Theobroma cacao (69% identity). Sequence and structural analysis indicated that PgPR6 belongs to class Kunitz-type PI family. This is the first report pertaining to the identification of PR6 gene from the P. ginseng genome. The high-level expression of PgPR6 was observed in root as revealed by quantitative real-time PCR. The temporal expression analysis demonstrated that PgPR6 expression was highly up-regulated by signaling molecules, heavy metals, mechanical wounding, chilling, salt, sucrose, and mannitol stress, indicating that PgPR6 may play an important role in the molecular defense response of ginseng to a various range of environmental stresses.
Авторлар туралы
D. Myagmarjav
Graduate School of Biotechnology and Ginseng Bank, College of Life Science
Email: yujinkim@khu.ac.kr
Корей Республикасы, Yongin, 446-701
J. Sukweenadhi
Graduate School of Biotechnology and Ginseng Bank, College of Life Science
Email: yujinkim@khu.ac.kr
Корей Республикасы, Yongin, 446-701
Y. Kim
Department of Oriental Medicinal Biotechnology, College of Life Science
Хат алмасуға жауапты Автор.
Email: yujinkim@khu.ac.kr
Корей Республикасы, Yongin, 446-701
M. Jang
Department of Oriental Medicinal Biotechnology, College of Life Science
Email: yujinkim@khu.ac.kr
Корей Республикасы, Yongin, 446-701
S. Rahimi
Graduate School of Biotechnology and Ginseng Bank, College of Life Science
Email: yujinkim@khu.ac.kr
Корей Республикасы, Yongin, 446-701
J. Silva
Graduate School of Biotechnology and Ginseng Bank, College of Life Science
Email: yujinkim@khu.ac.kr
Корей Республикасы, Yongin, 446-701
J. Choi
Department of Oriental Medicinal Biotechnology, College of Life Science
Email: yujinkim@khu.ac.kr
Корей Республикасы, Yongin, 446-701
P. Mohanan
Graduate School of Biotechnology and Ginseng Bank, College of Life Science
Email: yujinkim@khu.ac.kr
Корей Республикасы, Yongin, 446-701
W. Kwon
Department of Oriental Medicinal Biotechnology, College of Life Science
Email: yujinkim@khu.ac.kr
Корей Республикасы, Yongin, 446-701
C. Kim
Department of Oriental Medicinal Biotechnology, College of Life Science
Email: yujinkim@khu.ac.kr
Корей Республикасы, Yongin, 446-701
D.-C. Yang
Graduate School of Biotechnology and Ginseng Bank, College of Life Science; Department of Oriental Medicinal Biotechnology, College of Life Science
Email: yujinkim@khu.ac.kr
Корей Республикасы, Yongin, 446-701; Yongin, 446-701
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