Isolation of Polymorphic RAPD-SSR Markers from Xinjiang Arctic Grayling (Thymallus arcticus grubei) and a Test of Cross-Species Amplification
- Authors: Wang C.1, Liu Y.2, Liu L.3, Li Y.2
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Affiliations:
- College of Earth and Environment Science
- College of Life Sciences and Technology
- Linyi Academy of Agricultural Sciences
- Issue: Vol 54, No 5 (2018)
- Pages: 587-591
- Section: Short Communications
- URL: https://journals.rcsi.science/1022-7954/article/view/188989
- DOI: https://doi.org/10.1134/S1022795418050113
- ID: 188989
Cite item
Abstract
A total of 18 polymorphic microsatellite loci were isolated and characterized from RAPD products in the Xinjiang Arctic Grayling (Thymallus arcticus grubei). The number of alleles (Na) per locus varied from 2 to 10. Observed (Ho) and expected (He) heterozygosities ranged from 0.64 to 0.92, and from 0.63 to 0.88, respectively. Considerable differences were found among HBH, FH and FY populations in the number of alleles, effective number of alleles, number of genotypes at all of these loci. These new RAPD-SSR markers have provided a helpful tool for genetic analyses and resources conservation of T. arcticus grubei. Five additional fish species, Amur grayling (Thymallus grubii), Taimen (Hucho taimen), Sea perch (Lateolabrax japonicus), Lenok (Brachymystax lenok) and Red seam bream (Pagrosomus major) were assessed for cross-species amplification. Three of the five species showed at least one polymorphic locus. In addition, seven loci were found to be polymorphic in at least one species.
About the authors
C. Wang
College of Earth and Environment Science
Email: yguoliu@163.com
China, Huainan, 232001
Y.-G. Liu
College of Life Sciences and Technology
Author for correspondence.
Email: yguoliu@163.com
China, Urumqi, 830046
L.-X. Liu
Linyi Academy of Agricultural Sciences
Email: yguoliu@163.com
China, Linyi, 276012
Y.-Y. Li
College of Life Sciences and Technology
Email: yguoliu@163.com
China, Urumqi, 830046