Email this article Next-Generation Sequencing of Matched Ectopic and Eutopic Endometrium Identifies Novel Endometriosis-Related Genes
- Authors: Predeus A.V.1, Vashukova E.S.2,3, Glotov A.S.2,3, Danilova M.M.2, Osinovskaya N.S.2, Malysheva O.V.2,4, Shved N.Y.2, Ganbarli N.2, Yarmolinskaya M.I.2, Ivashchenko T.E.2, Baranov V.S.2
-
Affiliations:
- Bioinformatics Institute
- Ott Research Institute of Obstetrics, Gynecology and Reproductology
- Biobank of the Research Park, Saint Petersburg State University
- North-Western State Medical University named after I.I. Mechnikov
- Issue: Vol 54, No 11 (2018)
- Pages: 1358-1365
- Section: Medical Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/188679
- DOI: https://doi.org/10.1134/S1022795418110133
- ID: 188679
Cite item
Open Access
Access granted
Subscription Access
Abstract
Here, we determined basic differentially expressed genes in endometrial lesions compared to eutopic endometrium of the patients with endometriosis (EM). Endometrial biopsy and tissue sampling were performed in ten women with pelvic EM and nine controls at their mid-secretory phase. NGS of mRNA combined with bioinformatic analysis and complemented by RT-qPCR analysis were used for analysis. A list of differentially expressed genes of ectopic compared to eutopic endometrium during mid-secretary phase has been composed. Functional annotation has found profound gene expression perturbations in numerous EM pathways including extracellular matrix, complement and coagulation, as well as cell cycle-related gene family. The genes of alcohol dehydrogenase 1B (ADH1B) and fatty binding protein 4 (FABP4) were determined as the most dramatically up-regulated genes in ectopic endometrial lesions. Both genes were not reported before to be associated with EM. Several other genes including PLA2G2A were also up-regulated in ectopic EM lesions. Notably, FABP4 and ADH1B are key genes in basic metabolism and known for their function in adipose tissue. The results provide intriguing new avenue for endometriosis studies.
About the authors
A. V. Predeus
Bioinformatics Institute
Email: baranov@vb2475.spb.edu
Russian Federation, Saint Petersburg, 197342
E. S. Vashukova
Ott Research Institute of Obstetrics, Gynecology and Reproductology; Biobank of the Research Park, Saint Petersburg State University
Email: baranov@vb2475.spb.edu
Russian Federation, Saint Petersburg; Saint Petersburg
A. S. Glotov
Ott Research Institute of Obstetrics, Gynecology and Reproductology; Biobank of the Research Park, Saint Petersburg State University
Email: baranov@vb2475.spb.edu
Russian Federation, Saint Petersburg; Saint Petersburg
M. M. Danilova
Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: baranov@vb2475.spb.edu
Russian Federation, Saint Petersburg
N. S. Osinovskaya
Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: baranov@vb2475.spb.edu
Russian Federation, Saint Petersburg
O. V. Malysheva
Ott Research Institute of Obstetrics, Gynecology and Reproductology; North-Western State Medical University named after I.I. Mechnikov
Email: baranov@vb2475.spb.edu
Russian Federation, Saint Petersburg; Saint Petersburg
N. Yu. Shved
Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: baranov@vb2475.spb.edu
Russian Federation, Saint Petersburg
N. Ganbarli
Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: baranov@vb2475.spb.edu
Russian Federation, Saint Petersburg
M. I. Yarmolinskaya
Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: baranov@vb2475.spb.edu
Russian Federation, Saint Petersburg
T. E. Ivashchenko
Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: baranov@vb2475.spb.edu
Russian Federation, Saint Petersburg
V. S. Baranov
Ott Research Institute of Obstetrics, Gynecology and Reproductology
Author for correspondence.
Email: baranov@vb2475.spb.edu
Russian Federation, Saint Petersburg
