Comparative Estimate of the sheep breed gene pools using ISSR-analysis
- Authors: Nesteruk L.V.1, Makarova N.N.2, Evsyukov A.N.1, Svishcheva G.R.1,3, Lhasaranov B.B.4, Stolpovsky Y.A.1
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Affiliations:
- Vavilov Institute of General Genetics
- OOO AgriVolga
- Institute of Cytology and Genetics, Siberian Branch
- Shuluuma IAPC
- Issue: Vol 52, No 3 (2016)
- Pages: 304-313
- Section: Animal Genetics
- URL: https://journals.rcsi.science/1022-7954/article/view/187661
- DOI: https://doi.org/10.1134/S102279541603011X
- ID: 187661
Cite item
Abstract
Using the ISSR-PCR technique, the genetic structure of nine sheep breeds (Ovis aries) bred on the territories of Russia and Mongolia was examined. Species-specific and breed-specific DNA fragments were identified. For the first time, data on the genetic diversity of Telengit and Buubey sheep breeds were obtained. The main parameters of the genetic diversity and the breed structure were assessed, and the phylogenetic relationships and genetic distances between the studied breeds were determined. Using the method of hierarchical frequency averaging, the prototypal sheep gene pool was reconstructed. The three-tiered analysis of diversity based on the ISSR fingerprinting data showed that 15.8% of variability was found between the breeds, 31.4% of variability was found between the populations within the breeds, and the diversity among the individuals within the populations constituted 52.8%.
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About the authors
L. V. Nesteruk
Vavilov Institute of General Genetics
Author for correspondence.
Email: lubov-kas@mail.ru
Russian Federation, Moscow, 119991
N. N. Makarova
OOO AgriVolga
Email: lubov-kas@mail.ru
Russian Federation, Uglich, 152615
A. N. Evsyukov
Vavilov Institute of General Genetics
Email: lubov-kas@mail.ru
Russian Federation, Moscow, 119991
G. R. Svishcheva
Vavilov Institute of General Genetics; Institute of Cytology and Genetics, Siberian Branch
Email: lubov-kas@mail.ru
Russian Federation, Moscow, 119991; Novosibirsk, 630090
B. B. Lhasaranov
Shuluuma IAPC
Email: lubov-kas@mail.ru
Russian Federation, Buryatia, 671450
Yu. A. Stolpovsky
Vavilov Institute of General Genetics
Email: lubov-kas@mail.ru
Russian Federation, Moscow, 119991