Effect of Structural Disorder on Hydrodynamic Behavior of Alpha-Casein According to PFG NMR Spectroscopy
- Авторы: Kusova A.M.1,2, Sitnitsky A.E.1, Zuev Y.F.1,2,3
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Учреждения:
- Kazan Institute of Biochemistry and Biophysics, FSBIS KSC RAS
- Kazan (Volga Region) Federal University
- Kazan State Power Engineering University
- Выпуск: Том 49, № 5 (2018)
- Страницы: 499-509
- Раздел: Original Paper
- URL: https://journals.rcsi.science/0937-9347/article/view/248541
- DOI: https://doi.org/10.1007/s00723-018-0990-5
- ID: 248541
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Аннотация
The concentration dependences of self-diffusion coefficient for intrinsically disordered milk protein αS-casein were studied by pulsed field gradient nuclear magnetic resonance. The experimental data were analyzed in a view of phenomenological approach based on the frictional formalism of non-equilibrium thermodynamics by Vink. The results of αS-CN hydrodynamic study showed that at low- and high-protein concentrations, αS-CN exists in the different structural forms. At low concentrations in the rather broad concentration range, protein remains monomeric but with greater hydrodynamic size than have rigid globular proteins of the equal mass. At high concentrations beyond the definite protein content, αS-CN tends to form associates. The application of the Vink’s approach to αS-CN testifies that the role of flexible domains in the process of self-diffusion is mainly in increasing the friction of between αS-CN molecules due to their inter-entanglement. The latter physically means that when αS-CN molecules cling each other by their flexible domains, this phenomenon provides much more efficient friction than their interaction with solvent molecules.
Об авторах
A. Kusova
Kazan Institute of Biochemistry and Biophysics, FSBIS KSC RAS; Kazan (Volga Region) Federal University
Email: yufzuev@mail.ru
Россия, Lobachevsky Str., 2/31, Kazan; Kremlevskaya Str., 18, Kazan, 420008
A. Sitnitsky
Kazan Institute of Biochemistry and Biophysics, FSBIS KSC RAS
Email: yufzuev@mail.ru
Россия, Lobachevsky Str., 2/31, Kazan
Yu. Zuev
Kazan Institute of Biochemistry and Biophysics, FSBIS KSC RAS; Kazan (Volga Region) Federal University; Kazan State Power Engineering University
Автор, ответственный за переписку.
Email: yufzuev@mail.ru
ORCID iD: 0000-0002-6715-2530
Россия, Lobachevsky Str., 2/31, Kazan; Kremlevskaya Str., 18, Kazan, 420008; Krasnoselskaya Str., 51, Kazan, 420066
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