A Novel Approach to Interpretation of the Time-Dependent Self-Diffusion Coefficient: Water-Mouse RBCs Suspension Study
- Авторы: Loskutov V.1, Volkov V.2,3, Avilova I.2
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Учреждения:
- Physics Department, Mari State University
- Institute of Problems of Chemical Physics RAS
- Science Center in Chernogolovka RAS
- Выпуск: Том 50, № 1-3 (2019)
- Страницы: 305-321
- Раздел: Original Paper
- URL: https://journals.rcsi.science/0937-9347/article/view/248465
- DOI: https://doi.org/10.1007/s00723-018-1103-1
- ID: 248465
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Аннотация
Red Blood Cells (RBCs) are ideally suited as a model system for study of water diffusion in biological tissue. We used a pulsed-field-gradient nuclear magnetic resonance technique to measure the time-dependent self-diffusion coefficient D(t) in water-mouse RBC suspension at different diffusion times. A new method of interpretation of the experimental time dependence of the self-diffusion coefficient D(t) for fluid in heterogeneous media is proposed, and this study presents a new approximation describing water diffusion in such system. Three types of water were determined, which differs according to the self-diffusion coefficients: bulk water, extracellular and intracellular water. The water molecular exchange process among these three water states was investigated. The residence time for mice RBCs was obtained. Intracellular and extracellular water self-diffusion was restricted, and the sizes of restriction regions were 1.95 μm and 6.73 μm with S/V ratio 1.534 μm−1 and 0.44 μm−1, respectively. The cell wall permeability determined from the resident time as 4.7·10−5 m/s agreed with the permeability 5.4·10−5 m/s obtained from time dependence of intracellular water self-diffusion coefficient.
Об авторах
Valentin Loskutov
Physics Department, Mari State University
Автор, ответственный за переписку.
Email: val_losk@rambler.ru
ORCID iD: 0000-0002-9507-9246
Россия, Lenin Sq., 1, Mari El, Yoshka-Ola, 424000
Vitaly Volkov
Institute of Problems of Chemical Physics RAS; Science Center in Chernogolovka RAS
Email: val_losk@rambler.ru
Россия, Academician Semenov Avenue, 1, Moscow Region, Chernogolovka, 142432; Lesnaya Str.9, Moscow Region, Chernogolovka, 142432
Irina Avilova
Institute of Problems of Chemical Physics RAS
Email: val_losk@rambler.ru
Россия, Academician Semenov Avenue, 1, Moscow Region, Chernogolovka, 142432