Peptides on the Surface: Spin-Label EPR and PELDOR Study of Adsorption of the Antimicrobial Peptides Trichogin GA IV and Ampullosporin A on the Silica Nanoparticles
- Authors: Syryamina V.N.1,2, Samoilova R.I.1, Tsvetkov Y.D.1, Ischenko A.V.2,3, De Zotti M.4, Gobbo M.4,5, Toniolo C.4,5, Formaggio F.4,5, Dzuba S.A.1,2
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Affiliations:
- Institute of Chemical Kinetics and Combustion, RAS
- Novosibirsk State University
- Boreskov Institute of Catalysis, RAS
- Department of Chemical Sciences, University of Padova
- Institute of Biomolecular Chemistry, Padova Unit, CNR
- Issue: Vol 47, No 3 (2016)
- Pages: 309-320
- Section: Article
- URL: https://journals.rcsi.science/0937-9347/article/view/247439
- DOI: https://doi.org/10.1007/s00723-015-0745-5
- ID: 247439
Cite item
Abstract
The properties of antimicrobial peptides adsorbed on inorganic or organic surfaces are of interest for their potential applications in intracellular drug delivery. In this work, continuous-wave (CW) electron paramagnetic resonance (EPR) and pulsed electron-electron double resonance (PELDOR) techniques were applied to study adsorption of the short-sequence trichogin GA IV and the medium-length sequence ampullosporin A antimicrobial peptides on the monodisperse colloidal silica nanospheres of 20 nm diameter. The results obtained by CW EPR support the view that the adsorbed peptides form close-packed clusters. PELDOR data show that both trichogin and ampullosporin adsorbed on the silica surface possess a more disordered conformation as compared to that in solution. For ampullosporin, disordering is much more pronounced than for trichogin. After desorption, the peptides restored their conformations; upon adsorption the peptides in some cases may lose partly their biradical character.
About the authors
Victoria N. Syryamina
Institute of Chemical Kinetics and Combustion, RAS; Novosibirsk State University
Email: dzuba@kinetics.nsc.ru
Russian Federation, Novosibirsk, 630090; Novosibirsk, 630090
Rimma I. Samoilova
Institute of Chemical Kinetics and Combustion, RAS
Email: dzuba@kinetics.nsc.ru
Russian Federation, Novosibirsk, 630090
Yuri D. Tsvetkov
Institute of Chemical Kinetics and Combustion, RAS
Email: dzuba@kinetics.nsc.ru
Russian Federation, Novosibirsk, 630090
Arkady V. Ischenko
Novosibirsk State University; Boreskov Institute of Catalysis, RAS
Email: dzuba@kinetics.nsc.ru
Russian Federation, Novosibirsk, 630090; Novosibirsk, 630090
Marta De Zotti
Department of Chemical Sciences, University of Padova
Email: dzuba@kinetics.nsc.ru
Italy, Padua, 35131
Marina Gobbo
Department of Chemical Sciences, University of Padova; Institute of Biomolecular Chemistry, Padova Unit, CNR
Email: dzuba@kinetics.nsc.ru
Italy, Padua, 35131; Padua, 35131
Claudio Toniolo
Department of Chemical Sciences, University of Padova; Institute of Biomolecular Chemistry, Padova Unit, CNR
Email: dzuba@kinetics.nsc.ru
Italy, Padua, 35131; Padua, 35131
Fernando Formaggio
Department of Chemical Sciences, University of Padova; Institute of Biomolecular Chemistry, Padova Unit, CNR
Email: dzuba@kinetics.nsc.ru
Italy, Padua, 35131; Padua, 35131
Sergei A. Dzuba
Institute of Chemical Kinetics and Combustion, RAS; Novosibirsk State University
Author for correspondence.
Email: dzuba@kinetics.nsc.ru
Russian Federation, Novosibirsk, 630090; Novosibirsk, 630090
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