Cathepsin G in the immune defense of the human duodenum: New sources for biosynthesis
- 作者: Zamolodchikova T.1, Shcherbakov I.2, Khrennikov B.3, Shoibonov B.1, Svirshchevskaya E.4
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隶属关系:
- Anokhin Research Institute for Normal Physiology
- Gabrichevskii Research Institute of Epidemiology and Microbiology
- Infectious Clinical Hospital no. 1
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- 期: 卷 43, 编号 3 (2017)
- 页面: 326-333
- 栏目: Article
- URL: https://journals.rcsi.science/0362-1197/article/view/177197
- DOI: https://doi.org/10.1134/S0362119717020177
- ID: 177197
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详细
Proteases play a key role in the physiological processes of the small intestine, supporting its normal physiological functions as a part of the digestive system, in which hydrolysis and assimilation of nutrients are implemented. A high concentration of antigens in the intestinal lumen activates immunity and stimulates a chronic weakly expressed inflammatory response in a normal gastrointestinal tract (GIT). Cathepsin G, a serine protease controlling the functional state of immune cells, directly participates in the complicated system for the regulation of balance between physiological and pathological inflammations. To determine the role of cathepsin G in the small intestine, an immunofluorescent investigation of biopsies from the human duodenal mucosa were investigated using the confocal immunofluorescence microscopy method and human antibodies to cathepsin G. It has been shown for the first time that cathepsin G, which was regarded conventionally as one of the effectors of the inflammatory process, is a constitutive enzyme of the human duodenum and is constantly present in its normal mucosa. The new cell sources for the cathepsin G biosynthesis identified: intraepithelial lymphocytes (IELs), lamina propria lymphocytes, CD14-positive intestinal macrophages, and Paneth cells, which are specialized epitheliocytes of intestinal glands. Our data on the cathepsin G expression by immunocytes and Paneth cells in the duodenum allow us to attribute cathepsin G to the main proteases of intestinal immunity, which indicates the important role of this enzyme in the regulation of human GIT functions.
作者简介
T. Zamolodchikova
Anokhin Research Institute for Normal Physiology
编辑信件的主要联系方式.
Email: tatyanazam@yandex.ru
俄罗斯联邦, Moscow
I. Shcherbakov
Gabrichevskii Research Institute of Epidemiology and Microbiology
Email: tatyanazam@yandex.ru
俄罗斯联邦, Moscow
B. Khrennikov
Infectious Clinical Hospital no. 1
Email: tatyanazam@yandex.ru
俄罗斯联邦, Moscow
B. Shoibonov
Anokhin Research Institute for Normal Physiology
Email: tatyanazam@yandex.ru
俄罗斯联邦, Moscow
E. Svirshchevskaya
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: tatyanazam@yandex.ru
俄罗斯联邦, Moscow