Effect of vaginal microbiota composition in early pregnancy on pregnancy course and outcomes

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Objective: This study aimed to investigate the outcomes of pregnancy in relation to the quantitative and qualitative composition of cervicovaginal microbiota in early pregnancy using real-time polymerase chain reaction (RT-PCR).

Materials and methods: The study included 153 pregnant women at 7–12 weeks of gestation, with 73 experiencing a threatened miscarriage and 80 having a healthy pregnancy. The entire cohort was divided into three groups based on the outcomes. Group I (n=122) consisted of women who delivered at term, group II (n=7) included patients with preterm delivery, and group III (n=24) comprised women who experienced spontaneous miscarriage. The analysis involved laboratory investigation that quantitatively determined the DNA of opportunistic flora associated with the development of bacterial vaginosis (BV), aerobic vaginitis (AV), and candidal vulvovaginitis (CVV). Additionally, DNA from pathogens of four sexually transmitted infections (Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, and Trichomonas vaginalis) was determined. All study participants underwent diagnostic procedures in accordance with regulatory documents and received treatment in Moscow hospitals or outpatient settings, under the supervision of an obstetrician-gynecologist.

Results: Patients whose pregnancies were terminated in the first half of gestation had a higher incidence of BV (70.8%) than those who delivered at term (22.2%, p<0.05). The frequency of AV in women with spontaneous abortion was twice as high (8.3%) as that in patients who delivered at term (3.9%, p<0.05). The prevalence of VVC in the three groups was not statistically different. The presence of more than five microorganism markers involved in the development of BV, AV, and VVC, as well as opportunistic genital mycoplasmas detected in the vagina of a pregnant woman in the first trimester, was associated with the development of spontaneous miscarriage in the first trimester and preterm birth (p<0.01).

Conclusion: Laboratory evaluation of the reproductive tract using RT-PCR revealed higher detection rates of certain nosologies and syndromes in pregnant women with adverse pregnancy outcomes. The early detection of reproductive tract infections is associated with an increased risk of early pregnancy loss and preterm delivery.

Толық мәтін

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Авторлар туралы

Polina Shadrova

N.I. Pirogov Russian National Research Medical University, Ministry of Health of Russia; Higher School of Economics

Хат алмасуға жауапты Автор.
Email: shadrovapolina@hotmail.com
ORCID iD: 0000-0002-3721-1421
SPIN-код: 9935-8003

PhD, Teaching Assistant at the Department of Obstetrics and Gynecology of Medical Faculty

Ресей, Moscow; Moscow

Karina Bondarenko

Clinic of Modern Ozone Therapy

Email: karinabond@mail.ru
ORCID iD: 0000-0003-4147-1151
SPIN-код: 9976-0735

Dr. Med. Sci.

Ресей, Moscow

Alexander Guschin

Moscow Research and Practical Center of Dermatovenerology and Cosmetology

Email: mcdik@zdrav.mos.ru
ORCID iD: 0000-0002-0399-1167

PhD (Bio.), Leading Researcher

Ресей, Moscow

Alexander Zatevalov

G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology

Email: 89057149114@mail.ru

PhD (Bio.), Chief Researcher at the Laboratory of Diagnosis and Prevention of Infectious Diseases

Ресей, Moscow

Valeria Metalnikova

N.I. Pirogov Russian National Research Medical University, Ministry of Heath of Russia

Email: metalnikova-valeri@mail.ru
ORCID iD: 0000-0002-3432-7023

Resident at the Department of Obstetrics and Gynecology of Medical Faculty

Ресей, Moscow

Yulia Dobrokhotova

N.I. Pirogov Russian National Research Medical University, Ministry of Heath of Russia

Email: pr.dobrohotova@mail.ru
ORCID iD: 0000-0002-7830-2290

Dr. Med. Sci., Professor, Head of the Department of Obstetrics and Gynecology of Medical Faculty

Ресей, Moscow

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Қосымша файлдар

Қосымша файлдар
Әрекет
1. JATS XML
Жүктеу
2. Fig. 1. Distribution of the studied pregnant women

Жүктеу (98KB)
Метадеректер
3. Fig. 2. Prevalence of reproductive tract infections depending on pregnancy outcome

Жүктеу (77KB)
Метадеректер

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