Mast cells as predictors of bladder cancer

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Objective. To investigate mast cell populations in patients of different ages with Stage li-ill bladder cancer (BC), by using innovative immunohistochemical approaches (to study chymase and thymase of positive structures). Subjects and methods. Immunohistochemical approaches were used to examine material from 25 patients: a study group of 20 patients with histologically verified Stage ll-lll bladder cancer; a control group of 5 men aged 36-50years without cancer who died in traffic accidents. Results. The investigation ofthe protease profile of a mast cell population in the bladder showed that during the process of cancer development, the time course of changes In the content of protease-posltive mast cells was different: the number of tryptase-positlve mast cells Increased, while that of chymase-positive mast cells significantly decreased. An analysis ofthe protease profile indicated that with the process of cancer development, mast cell chymase expression noticeably decreased; the size of mast cells in the connective tissue subpopulation reduced. However, they could form local intraorganlc accumulations, secreting both tryptase and chymase in fairly immense areas.

作者简介

T. Pavlova

Belgorod State National Research University

编辑信件的主要联系方式.
Email: pavlova@bsu.edu.ru

доктор медицинских наук, профессор

D. Atyakshin

N.N. Burdenko Voronezh State Medical University

Email: pavlova@bsu.edu.ru

Research Institute of Experimental Biology and Medicine

I. Pavlov

Belgorod State National Research University

Email: pavlova@bsu.edu.ru

кандидат медицинских наук

D. Bessmertny

Belgorod State National Research University

Email: pavlova@bsu.edu.ru

кандидат медицинских наук

N. Pilkevich

Belgorod State National Research University

Email: pavlova@bsu.edu.ru

доктор медицинских наук, профессор

K. Proshchaev

Belgorod State National Research University

Email: pavlova@bsu.edu.ru

доктор медицинских наук, профессор

参考

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  2. Онкогеронтология. Руководство для врачей. Под ред. В.Н. Анисимова, А.М. Беляева. СПб: Издательство AHHM0 «Вопросы онкологии», 2017; 509.
  3. Злокачественные новообразования в России в 2020 года (заболеваемость и смертность). Под ред. А.Д. Каприна, В.В. Старинского, А.О. Шахзадовой. М.: МНИОИ им. П.А. Герцена - филиал ФГБУ «НМИЦ радиологии» Минздрава России, 2021; 252 с.
  4. Павлова Т.В., Пилькевич Н.Б., Бессмертный Д.В. и др. Особенности метаболического атилизма при развитии онкологической патологии мочеполовой системы. Молекулярная медицина. 2021; 19 (31): 30-4. D01:10.29296/24999490-2021 -01-04
  5. Клинические рекомендации. Рак мочевого пузыря. Кодирование по Международной статистической классификации болезней и проблем, связанных со здоровьем: С67. Возрастная группа: взрослые. 2020; с. 81.
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  8. Buchwalow, I.B., Boecker W. Immunohistochemistry: Basics and Methods, 19 ed. Berlin, Heidelberg (Germany), Dordrecht (Netherlands), London (UK), New York (USA): Springer, 2010; 152 p.
  9. Atiakshin D.A., Shishkina V.V., Gerasimova O.A. et al.Combined histochemical approach in assessing tryptase expression in the mast cell population. Acta Histochem. 2021; 123:151-71. doi: 10.1016/j.acthis.2021.151711

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2. Fig. 1. Transitional cell BC (a male patient aged 55 years); the tissue consists of cells with impaired polarity, which have lost intercellular contacts with large hyperchromatic nuclei; there are commonly mitotic figures that are often atypical; H&E; Fragment One: a – ×250; б – ×100; Fragment Two: в – ×100; г – ×400

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3. Fig. 2. Bladder fragments in the control group: a, б – a male patient aged 40 years; the location of mast cells in the endomysium; the pericapillary location in the bladder muscle layer; active granular secretion (б – the protease profile of a mast cell population [tryptase]); в, г – a male patient aged 45 years

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4. Fig. 3. A bladder fragment in BC: a, б – a male patient aged 50 years; the mast cells are located in the area of the bladder stroma, as well as the basement membrane of the atypical bladder epithelium; active tryptase secretion; there are a large number of secretory granules; secretion of individual tryptase-positive granules into the extracellular matrix; selective secretion of mast cell tryptase to the stromal cell nucleus (б – the protease profile of a mast cell population [tryptase]); в, г – a female patient aged 65 years; chymase-positive mast cells are located in the peritumoral area of the bladder stroma; the high content of chymase-positive granules in the mast cell cytoplasm (г – the protease profile of a mast cell population [chymase])

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5. Fig. 4. Mast cells in bladder tissue (tryptase (green), chymase (red), DAPI staining (blue): a – a control group: a male patient aged 45 years; a mast cell with simultaneous content of tryptase and chymase; б – a study group: a male patient aged 50 years with BC; there is a mast cell with small granules, with simultaneous expression of chymase and tryptase; multiple immunolabeling of mast cell tryptase and chymase

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