Membrane and Cell BiologyMembrane and Cell BiologyБиологические мембраны0233-47553034-5219The Russian Academy of Sciences40597610.7868/S3034521926010031ArticlesСТАТЬИResearch ArticleThe Role of Lipid Rafts in the Regulation of Piezo1 Channels in C2C12 MyoblastsРоль липидных рафтов в регуляции каналов Piezo1 в миобластах C2C12BildyugN. BБильдюгН. Бnbildyug@gmail.comVasilevaV. YВасильеваВ. Ю-ShenkmanB. SШенкманБ. С-MirzoevT. MМирзоевТ. Мtmirzoev@yandex.ruInstitute of Cytology of the Russian Academy of SciencesИнститут цитологии РАНInstitute of Biomedical Problems of the Russian Academy of SciencesИнститут медико-биологических проблем РАН15022026431VOL 43, NO1 ()ТОМ 43, №1 ()263903042026Copyright ©; 2026, Russian Academy of SciencesCopyright ©; 2026, Российская академия наук2026Russian Academy of SciencesРоссийская академия наукhttps://journals.rcsi.science/0233-4755/article/view/405976

Piezo1 channels are mechanically activated (MA) cation channels that play an important role in skeletal muscle physiology. However, the specific mechanisms of Piezo1 regulation in skeletal muscle are not yet fully understood. At the same time, the activity of different ion channels is known to be dependent on lipid rafts, which are dynamic microdomains in the cell membrane enriched in sphingolipids and cholesterol. Therefore, the aim of this study was to evaluate the role of lipid rafts in the muscle-specific regulation of Piezo1 using a cholesterol-removing agent, methyl-beta-cyclodextrin (MβCD), and the sphingolipid sphingosine-1-phosphate (S1P) in a C2C12 myoblast model. Fluorescence labeling and intracellular calcium measurements demonstrated that the disruption of lipid rafts with MβCD resulted in disassembly of the initially present actin cytoskeleton in C2C12 cells and decreased Piezo1-mediated Ca2+ influx into the cells. In contrast, stimulation of myoblasts with S1P resulted in increased formation of lipid rafts and actin stress fibers, as well as enhanced Ca2+ influx through Piezo1 channels. These findings suggest the involvement of lipid rafts in the regulation of Piezo1 activity in C2C12 myoblasts, which may be mediated by lipid raft components themselves and/or by lipid raft-induced rearrangements of the actin cytoskeleton.

Каналы Piezo1 представляют собой механоактивируемые (МА) катионные каналы, которые играют важную роль в физиологии скелетных мышц. Однако конкретные механизмы регуляции Piezo1 в мышечной ткани остаются не изученными. В то же время известно, что активность различных ионных каналов зависит от липидных рафтов, которые представляют собой динамические микродомены плазматической мембраны клеток, обогащенные сфинголипидами и холестерином. Целью данного исследования являлась оценка роли липидных рафтов в регуляции Piezo1 в скелетных мышцах на модели мышиных миобластов C2C12 с использованием холестерин-секвестрирующего агента – метил-бета-циклодекстрина (МЦД) – и сфинголипида сфингозин-1-фосфата (S1P). Результаты флуоресцентного окрашивания и измерения внутриклеточного кальция показали, что разрушение липидных рафтов с помощью МЦД приводило к разборке активного цитоскелета в клетках C2C12 и снижению опосредованного Piezo1 притока Ca2+. S1P, напротив, стимулировал образование липидных рафтов и активных стресс-фибрилл в этих клетках и вызывал усиление притока Ca2+ через каналы Piezo1. Полученные результаты свидетельствуют об участии липидных рафтов в регуляции активности Piezo1 в миобластах C2C12, которая может быть опосредована компонентами липидных рафтов и/или зависимой от липидных рафтов перестройкой активного цитоскелета.

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