Cells of Azulene-Containing Plants: Spectral Studies

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Abstract

The spectral study of the surface and isolated organelles of cells of azulene-containing plants of temperate climate, dry and humid subtropics of Russia was carried out. The presence of azulenes in leaves, flower petals, nuclei, and chloroplasts was detected, which was assessed by the appearance of blue color and characteristic maxima in the region of 580–640 nm in the absorbance spectra and 405–430 nm in the fluorescence spectra. These data were confirmed in experiments on extracts of these hydrophobic pigments obtained with acetone or ethanol. The pigments were extracted from the surface and from the inside of the leaves after infusing of intact leaves or isolated organelles (nuclei and chloroplasts) for 10 min or 24 h, respectively, then chromatographic purification of the azulene fraction from chlorophyll was done. The results obtained may be of interest for cellular monitoring of azulene-containing plants that may be useful for pharmacology.

About the authors

V. V Roshchina

Institute of Cell Biophysics, Russian Academy of Sciences, FRC PSCBR RAS

Email: roshchinavic@mail.ru
Pushchino, Russia

G. A Soltani

National Park

Sochi, Russia

E. S Kraynyuk

Nikitsky Botanical Garden of the Russian Academy of Sciences

Yalta, Russia

M. M Shovkun

Prioksko-Terrasny Reserve

Danki, Serpukhov region, Russia

V. E Demidov

Prioksko-Terrasny Reserve

Danki, Serpukhov region, Russia

References

  1. Рощина В.В., Яшин В.А., Кучин А.В., Куньев А.Р., Солтани Г.А., Хайбулаева Л.М., Призова Н.К. 2022. Присутствие азуленов на поверхности растительных клеток как тест на чувствительность к озону. Биол. мембраны. 39 (1), 54–62. https://www.doi.org/10.31857/S023347552201008X
  2. Рощина В.В., Призова Н.К., Хайбулаева Л.М. 2022. Азулены листовой поверхности как защитный оптический фильтр. Актуальные вопросы биологической физики и химии. 7 (1), 36–39. https://www.doi.org/10.29039/rusjbpc.2022.0480
  3. Коновалов Д.А. 1995. Природные азулены. Растительные ресурсы. 31 (1), 101–130.
  4. Bakun P., Czarczynska-Goslinska B., Goslinski T., Lijewski S. 2021. In vitro and in vivo biological activities of azulene derivatives with potential applications in medicine. Med. Chem. Res. 30, 834–846. https://www.doi.org/10.1007/s00044-021-02701
  5. Murfin L.C., Lewis S.E. 2021. Azulene – a Bright core fore sensing and imaging. Molecules. 26 (2), 353–362. https://www.doi.org/10.3390/molecules26020353
  6. Рощина В.В., Сергиевич Л.А. 2024. Исследование влияния азуленов как защитных факторов на жизнеспособность мышей. Актуальные вопросы биологической физики и химии. 9 (2), 168–173.
  7. Roshchina V.V., Shovkun M.M., Demidov V.E. 2024. Spectral search of azulene-containing plants of temperate climate as possible new biological resources for pharmacy. J. Med. Plant Herbs. 3 (3), 1–14.
  8. Roshchina V.V., Kraynyuk E.S. 2025. Spectral studies for the search for azulene-containing plants in dry subtropics of the Crimea. J. Plant Biol. Agron. 4, 1–14.
  9. Roshchina V.V., Soltani G.A. 2025. The study of seasonal changes of azulenes in Eucalyptus cinerea with spectral methods. Allelopathy J. 66 (1), 59–68.
  10. Roshchina V.V. 2022. Possible role of azulene in plant life: Experiments with models. SMP Environ. Sci. Technol. 1, 1–10.
  11. Рощина В.В., Яшин В.А. Куньев А.Р. 2023. Исcледование спектральных характеристик поверхности растительной клетки: присутствие азуленов и биогенных аминов. Биол. мембраны. 40 (5), 351–361. https://www.doi.org/10.31857/S0233475523050079
  12. Roshchina V.V. 2023. Plant leaf surface as a sensory system in allelopathic relations: 1. Role of azulenes. Allelopathy J. 59 (2), 109–122. https://www.doi.org/10.26651/allelo.j/2023-59-2-1435
  13. Roshchina V.V. 1999. Mechanisms of cell-cell communication. In: Allelopathy Update. Ed. Narwal S.S. Vol. 2. Basic and Applied Aspects. Enfield (USA): Science Publisher, p. 4–24.
  14. Roshchina V.V. 2024. Azulenes in plant cell: Clover as their useful resource. Ann. Agricultur. Crop Scien. 9 (1), 02–07. https://www.doi.org/10.26420/ANNAGRICCROPSCI.2024.1147
  15. Robinson S.P., Edvards G.E., Walker D.A. 1979. Established methods for the isolation of intact chloroplasts. In: Plant Organelles. Ed. Reid E. Chichester: Ellis Horwood, p. 13–24.
  16. Рощина В.В. 2006. Хемосигнализация в клетках растительных микроспор. Известия РАН. Серия биол. 4, 414–420.
  17. Рощина В.В., Мельникова Е.В., Спиридонов Н.А., Ковалева Л.В. 1995. Азулены – синие пигменты пыльцы. Доклады РАН. 340 (5), 715–718.
  18. Рощина В.В., Мельникова Е.В., Яшин В.А., Карнаухов В.Н. 2002. Автофлуоресценция интактных спор хвоща Equisetum arvense L. в процессе развития. Биофизика. 47 (2), 318–324.
  19. Рощина В.В., Куньев А.Р, Фатерыга В.В., Шовкун М.М. 2023. Применение микроспектрофлуориметра/микроспектрофотометра для исследования поверхности растительных клеток. Актуальные вопросы биологической физики и химии. 8 (3), 137–142.
  20. Золотарев В.М. 2012. Применение дифференцирования в спектроскопии отражения. Оптика и спектроскопия. 112 (1), 150–154.
  21. Roshchina V.V. 2004. Cellular models to study the al- lelopathic mechanisms. Allelopathy J. 13 (1), 3–16.
  22. Roshchina V.V., Yashin V.A., Yashina A.V., Goltyaev M.V. 2011. Colored allelochemicals in modelling of cell-cell al- lelopathic interactions. Allelopathy J. 28 (1), 1–12.
  23. Heilbronner E. 1959. Azulenes. In: Non-benzenoid aromatic compounds. Ed. D. Ginsburg. New York, London: Intersci. Publ., p. 171–276.
  24. Рощина В.В., Мельникова Е., Гордон Р.Я., Коновалов Д.А., Кузин А.М. 1998. Исследование радиопротекторного действия проазуленов на хемосенсорной модели пыльцы Hippeastrum hybridum. Доклады РАН. 348 (4), 548–551.
  25. Ueki J.-I., Sarfgami H., Wakabayashi H. 2013. Anti-UV activity of newly-synthesized water-soluble azulenes. In vivo. 27 (1), 119–126.
  26. Matěnová M., Lorelei Horhoiu V., Dang F.X., Pospíšil P., Alster J., Burda J.V., Balaban T.S., Pšenčík J. 2014. Energy transfer in aggregates of bacteriochlorophyll c self-assembled with azulene derivatives. Phys. Chem. Chem. Phys. 16 (31), 16755–16764. https://www.doi.org/10.1039/c4cp01311e
  27. Lash T.D., Hayes M.J. 1997. Carbaporphyrins. Angew. Chem., Int. Ed. Engl. 36, 840–842. https://www.doi.org/10.1002/anie.199708401
  28. Lash T.D. 2016. Out of the Blue! Azuliporphyrins and related carbaporrhynoid systems. Acc. Chem. Res. 49 (3), 471–482. https://www.doi.org/10.1021/acs.accounts.5b00523
  29. Lash T.D. 2023. Organometallic chemistry within the structured environment provided by the macrocyclic cores of carbaporphyrins and related systems. Molecules. 28 (3), 1496. https://www.doi.org/10.3390/molecules28031496
  30. Lash T.D., Chaney S.T. 1997. Azuliporphyrin, a case of borderline porphyrinoid aromaticity. Angew. Chem., Int. Ed. Engl. 36, 839–840.
  31. Rajput S.S., Raghuvanshi N., Banana T., Yadav P., Alam M. 2024. Why does the orientation of azulene affect the two-photon activity of a porphyrinoid–azulene system? Phys. Chem., Chem. Phys. 26, 15611–15619.

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