Development of the St/J and V Genome Specific Molecular Marker Based on 5s rDNA Polymorphism in Thinopyrum bessarabicum, Pseudoroegneria spicata, and Dasypyrum villosum


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Nontranscribed spacers (NTS) of 5S rDNA are often polymorphic in closely related species and even in the same genome. The polymorphism of 5S rDNA NTS was shown between genomes St, J, and V of Triticeae species Thinopyrum bessarabicum, Pseudoroegneria spicata, and Dasypyrum villosum, respectively. A molecular genetic marker was designed based on the 5S rDNA NTS polymorphism that allows identification of the St, J, and V genomes. We designed a pair of primers that correspond to the conserved regions of 5S rDNA NTS between the genomes studied. The PCR amplicon length is 158 bp, 171 bp, and 172 bp for V, St, and J genomes, respectively. The fragment of the St genome is characterized by the SmiM I restriction site that enables its differentiation from the J genome fragment that lacks this site. The developed marker showed its efficiency for verification of germplasm accessions and the study of allopolyploids.

作者简介

O. Alexandrov

Center for Molecular Biotechnology

编辑信件的主要联系方式.
Email: olegsandrov@gmail.com
俄罗斯联邦, Moscow, 127550

M. Divashuk

Center for Molecular Biotechnology; All-Russia Research Institute of Agricultural Biotechnology

Email: olegsandrov@gmail.com
俄罗斯联邦, Moscow, 127550; Moscow, 12755

G. Karlov

Center for Molecular Biotechnology; All-Russia Research Institute of Agricultural Biotechnology

Email: olegsandrov@gmail.com
俄罗斯联邦, Moscow, 127550; Moscow, 12755

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