Adaptive variability of blood-forming elements in the annual life cycle of ground squirrels Urocitellus undulatus

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

In this study, changes in all blood formed elements of long-tailed ground squirrel Urocitellus undulatus (n=100) during different periods of their life activity were investigated. The hematological analysis was performed on an automatic veterinary analyser: a indices of leukocytes, platelets and erythrocytes were measured in summer (normothermia, june-july), autumn (preparatory period, october), winter torpid (hypothermia, december-february) and winter active periods (IBA, euthermia, short awakenings between hypothermia stages, december-february). It was shown that the total counts of platelets and leukocytes of ground squirrel increased by ~40 % in autumn compared to summer. During the torpor period, thrombocytosis and leukocytosis were replaced by extreme thrombocytopenia (~90 %) and leukopenia (~80 %). On awakening, the indices reached “summer” values, except for a slightly increased mean platelets volume. In autumn, an insignificant erythrocytosis (~10 %) was observed, which, together with changes in other parameters, could indicate an unexpressed autumn hypoxia. In torpor and in winter euthermia, the erythrocyte indices were no difference with summer control values; however, the presence of atypical forms of erythrocytes was detected both in the autumn period of preparation for hibernation and during hibernation. The results are discussed in the context of the adaptation of the blood-forming elements of the ground squirrel to the extreme conditions of hibernation and are valuable for the study of the adaptive abilities of homoeothermic animals and humans.

Толық мәтін

Рұқсат жабық

Авторлар туралы

P. Teplova

Institute of Cell Biophysics, Russian Academy of Sciences

Хат алмасуға жауапты Автор.
Email: p.o.teplova@gmail.com
Ресей, Pushchino

N. Komelina

Institute of Cell Biophysics, Russian Academy of Sciences

Email: p.o.teplova@gmail.com
Ресей, Pushchino

A. Yegorov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: p.o.teplova@gmail.com
Ресей, Pushchino

K. Lizorkina

Institute of Cell Biophysics, Russian Academy of Sciences

Email: p.o.teplova@gmail.com
Ресей, Pushchino

N. Zakharova

Institute of Cell Biophysics, Russian Academy of Sciences

Email: p.o.teplova@gmail.com
Ресей, Pushchino

Әдебиет тізімі

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2. Fig. 1. Seasonal differences in long-tailed ground squirrels Urocitellus undulatus (by blood parameters) in the space of principal components PC1 and PC2. The percentage of variation in the original variables explained by the corresponding principal component is given. (a) – all groups of animals, (b) – comparison of summer and autumn animals, (c) – comparison of animals in torpor and during winter activity, (d) – comparison of summer and winter active ground squirrels. Designations of points: green circle – summer animals, orange triangle – autumn group, blue star – ground squirrels in torpor, light blue square – IBA, winter activity. Each point describes one ground squirrel by 15 hematological indices, n = 25 for each group (see methods).

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3. Fig. 2. Load graph obtained as a result of principal component analysis of blood parameters of the long-tailed ground squirrel Urocitellus undulatus.

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4. Fig. 3. Seasonal changes in the leukocyte pool in the peripheral blood of U. undulatus ground squirrels (n = 25 for each season). Values ​​are presented as a box plot, where ● are outliers, * are statistically significant differences with the “summer” group (p <0.05, here and below the Mann–Whitney U test), ns are statistically significant differences (p ≥0.05). WBC – leukocytes; Mon – monocytes; Lymph – lymphocytes; Gran – granulocytes (total neutrophils, basophils and eosinophils).

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5. Fig. 4. Seasonal changes in platelets in the peripheral blood of U. undulatus ground squirrels (n = 25 for each season). Values ​​are presented as a box plot, where ● are outliers, * are statistically significant differences between groups (p < 0.05), ns are statistically significant differences (p ≥ 0.05). PLT is the platelet count; PCT is the platelet crit; MPV is the mean platelet volume; PDW is the platelet distribution width by volume.

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6. Fig. 5. Seasonal changes in erythrocyte parameters in the peripheral blood of U. undulatus ground squirrels (n = 25 for each season). Values ​​are presented as a box plot, where ● are outliers, * are statistically significant differences between groups (p <0.05), ns are statistically significant differences (p ≥0.05). RBC – erythrocytes; HGB – hemoglobin concentration; HCT – hematocrit; MCV – mean corpuscular volume; MCHC – mean corpuscular hemoglobin concentration; RDW – erythrocyte distribution width.

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