Expression of DROSHA and DICER genes in peripheral blood leukocytes in lung sarcoidosis
- 作者: Malysheva I1, Balan O1, Tikhonovich E2, Volkova T3
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隶属关系:
- IB KarRC RAS, laboratory of genetics
- Republican Hospital named after. V.A. Baranov
- Petrozavodsk state University, Department of Hospital Therapy
- 期: 卷 90, 编号 3 (2018)
- 页面: 21-24
- 栏目: Editorial
- URL: https://journals.rcsi.science/0040-3660/article/view/32720
- DOI: https://doi.org/10.26442/terarkh201890321-24
- ID: 32720
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详细
Aim. To study the expression level of the genes DROSHA and DICER in peripheral blood leukocytes (PBL) of patients with sarcoidosis of the lungs Materials and methods. The study included 32 patients diagnosed with persistent lung sarcoidosis (mean age 41.56±1.27 years) and 36 healthy donors (control; mean age 42.79±1.95 years). The level of expression of messenger RNA (mRNA) of the genes DROSHA and DICER were determined in PBL of healthy donors and patients with sarcoidosis of the lung by polymerase chain reaction in real time. Results. As a result of the conducted researches it is established that the level of drosha gene expression in PBL patients with sarcoidosis of lungs is significantly reduced in comparison with the control (p<0.01). We also found a significant decrease in the number of Dicker gene transcripts in the PBL of the study group of patients (p<0.01). Conclusion. According to the results of the conducted studies, a significant decrease in the number of DROSHA and DICER transcripts in PBL patients with the development of lung sarcoidosis has been found, which can contribute to the pathogenesis of this disease.
作者简介
I Malysheva
IB KarRC RAS, laboratory of genetics
Email: i.e.malysheva@yandex.ru
с.н.с. лаборатории генетики Petrozavodsk, Russia
O Balan
IB KarRC RAS, laboratory of geneticsс.н.с. лаборатории генетики Petrozavodsk, Russia
E Tikhonovich
Republican Hospital named after. V.A. Baranovзав. отд-нием респираторной терапии Petrozavodsk, Russia
T Volkova
Petrozavodsk state University, Department of Hospital Therapyд.б.н., проф. Petrozavodsk, Russia
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