Obtaining DNA aptamers to human interleukin-6 for biomagnetic immunoassay nanosensors
- Авторы: Spiridonova V.A.1, Novikova T.M.1, Snigirev O.V.2
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Учреждения:
- Belozersky Institute of Physicochemical Biology
- Department of Physics
- Выпуск: Том 71, № 1 (2016)
- Страницы: 135-138
- Раздел: Biophysics and Medical Physics
- URL: https://journals.rcsi.science/0027-1349/article/view/164435
- DOI: https://doi.org/10.3103/S002713491601015X
- ID: 164435
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Аннотация
We used aptamers, which are functional equivalents of antibodies, in order to develop a nanosensor immunoassay system based on magnetic nanoparticles and a SQUID magnetometer. Selection was used to obtain DNA aptamers to interleukin-6; their affinity to the target protein was characterized by surface plasmon resonance. It was shown that the biotinylated aptamer binds to magnetic nanoparticles that were functionalized with streptavidin.
Об авторах
V. Spiridonova
Belozersky Institute of Physicochemical Biology
Email: osnig@inbox.ru
Россия, Moscow, 119991
T. Novikova
Belozersky Institute of Physicochemical Biology
Email: osnig@inbox.ru
Россия, Moscow, 119991
O. Snigirev
Department of Physics
Автор, ответственный за переписку.
Email: osnig@inbox.ru
Россия, Moscow, 119991
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