Email this article Multicopper Oxidase-Catalyzed Biotransformation of Dihydroquercetin
- Authors: Khlupova M.E.1, Vasil’eva I.S.1, Shumakovich G.P.1, Morozova O.V.1, Zaitseva E.A.2, Chertkov V.A.3, Shestakova A.K.4, Kisin A.V.4, Yaropolov A.I.1
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Affiliations:
- Bach Institute of Biochemistry, Research Center of Biotechnology
- Department of Enzymology, Faculty of Chemistry
- Department of Organic Chemistry, Faculty of Chemistry
- State Research Institute of Chemistry and Technology of Organoelement Compounds
- Issue: Vol 73, No 5 (2018)
- Pages: 237-243
- Section: Article
- URL: https://journals.rcsi.science/0027-1314/article/view/163741
- DOI: https://doi.org/10.3103/S002713141805005X
- ID: 163741
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Abstract
Multicopper oxidases such as bilirubin oxidase (BOD) from Myrothecium verrucaria and laccase (LC) from the basidial fungus Trametes hirsuta have been used as catalysts in dihydroquercetin (DHQ) oxidative polymerization. The conditions selected enabled good yields of DHQ oligomers, which were then analyzed using UV-vis, FTIR, 1Н and 13С NMR spectroscopy. DHQ oligomers synthesized using both enzymes showed higher thermostability as compared with the monomer. Depending on the oxidase, the products of DHQ polymerization differed in physicochemical properties, and as shown by NMR studies, had different structures.
About the authors
M. E. Khlupova
Bach Institute of Biochemistry, Research Center of Biotechnology
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
I. S. Vasil’eva
Bach Institute of Biochemistry, Research Center of Biotechnology
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
G. P. Shumakovich
Bach Institute of Biochemistry, Research Center of Biotechnology
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
O. V. Morozova
Bach Institute of Biochemistry, Research Center of Biotechnology
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
E. A. Zaitseva
Department of Enzymology, Faculty of Chemistry
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119991
V. A. Chertkov
Department of Organic Chemistry, Faculty of Chemistry
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119991
A. K. Shestakova
State Research Institute of Chemistry and Technology of Organoelement Compounds
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119334
A. V. Kisin
State Research Institute of Chemistry and Technology of Organoelement Compounds
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119334
A. I. Yaropolov
Bach Institute of Biochemistry, Research Center of Biotechnology
Author for correspondence.
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
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