SIVA1 Regulates the Stability of Single-Stranded DNA-Binding Protein 3 Isoforms

Z. Yin; K. Zhang; X. Peng; Z. Jiang; W. Yuan; Y. Wang; Y. Li; X. Ye; Y. Dong; Y. Wan; B. Ni; P. Zhu; X. Fan; X. Wu; X. Mo

doi:10.1134/S0026893318050163

SIVA1 Regulates the Stability of Single-Stranded DNA-Binding Protein 3 Isoforms

作者: Yin Z.¹^,2, Zhang K.³, Peng X.¹, Jiang Z.¹, Yuan W.¹, Wang Y.¹, Li Y.¹, Ye X.¹, Dong Y.¹, Wan Y.¹, Ni B.², Zhu P.⁴, Fan X.¹, Wu X.¹, Mo X.¹
隶属关系:
1. The Center for Heart Development, State Key Lab Developmental Biology of Freshwater Fish, The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, Key Laboratory of Physical Fitness and Exercise, Rehabilitation of Hunan Province, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University
2. Birth Health and Genetics Lab, Parenthood Research Institute of Hunan Province
3. Deptment of Geriatrics, Xiangya Hospital, Central South University
4. Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences
期: 卷 52, 编号 5 (2018)
页面: 707-714
栏目: Molecular Cell Biology
URL: https://journals.rcsi.science/0026-8933/article/view/163682
DOI: https://doi.org/10.1134/S0026893318050163
ID: 163682

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详细

The assembly of LIM-homeodomain (LIM-HD) transcriptional complex plays important roles in early neuronal development. The stability of LIM-HD is controlled by single-strand binding protein 3 (SSBP3) via a cascade mechanism protecting it from proteasomal degradation. The expression level of SSBP3 has to be precisely regulated. Although a decrease of SSBP3 level is associated with several diseases, the mechanism of SSBP3 downregulation and whether SSBP3 itself is subject to proteasomal degradation remain largely unknown. Two strongly conserved transcripts of the SSBP3 gene, SSBP3a and SSBP3c, were cloned from a human brain cDNA library. By RT-PCR, we show that Ssbp3c is continuously expressed in both embryonic and adult mouse brain, whereas Ssbp3a is restricted to embryonic brain tissue. By co-IP and GST pulldown assays, we identified SIVA1 as a novel SSBP3-binding factor. In a ubiquitination assay, we show that SIVA1 enhances the ubiquitination of SSBP3 and regulates its abundance. Our findings reveal the proteasomal degradation of SSBP3 for the first time and provide a rationale for an SIVA1-SSBP3-dependent mechanism for the disassembly of LIM-HD multiprotein complexes.

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SSBP3, SIVA1, alternative splicing, protein interaction, ubiquitination

作者简介

Z. Yin

The Center for Heart Development, State Key Lab Developmental Biology of Freshwater Fish,
The National & Local Joint Engineering Laboratory of Animal Peptide Drug Development, Key Laboratory of Physical Fitness and Exercise, Rehabilitation of Hunan Province, Key Lab of MOE for Development Biology and Protein Chemistry,
College of Life Sciences, Hunan Normal University; Birth Health and Genetics Lab, Parenthood Research Institute of Hunan Province

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Email: fansha1234@aliyun.com
中国, Changsha, Hunan, 410081; Changsha, Hunan Province

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