Enviar artigo por via de e-mail The Novel Short Isoform of Securin Stimulates the Expression of Cyclin D3 and Angiogenesis Factors VEGFA and FGF2, but Does Not Affect the Expression of MYC Transcription Factor
- Autores: Demin D.E.1,2, Bogolyubova A.V.1,3, Zlenko D.V.3, Uvarova A.N.1,3, Deikin A.V.4, Putlyaeva L.V.1, Belousov P.V.1, Mitkin N.A.1, Korneev K.V.1,3, Sviryaeva E.N.1, Kulakovskiy I.V.1,5, Tatosyan K.A.1, Kuprash D.V.1,2,3, Schwartz A.M.1,2
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Afiliações:
- Engelhardt Institute of Molecular Biology
- Moscow Institute of Physics and Technology
- Faculty of Biology
- Institute of Gene Biology
- Vavilov Institute of General Genetics
- Edição: Volume 52, Nº 3 (2018)
- Páginas: 436-445
- Seção: Molecular Cell Biology
- URL: https://journals.rcsi.science/0026-8933/article/view/163552
- DOI: https://doi.org/10.1134/S0026893318030032
- ID: 163552
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Resumo
Pituitary tumor-transforming gene-1 (PTTG1) encodes securin, a multifunctional protein involved in development of various types of cancer. Securin participates in the regulation of sister chromatids separation and the expression of multiple genes involved in the control of the cell cycle, metabolism, and angiogenesis. In several human cell lines, we have found a novel short isoform of securin mRNA, which does not contain exons 3 and 4. After the translation of this new mRNA, a shortened protein is produced that, like the full-size form, is able to activate the transcription of cyclin D3 gene (CCND3), which controls the G1/S transition and angiogenesis factors VEGFA (vascular endothelial growth factor), and FGF2 (fibroblast growth factor 2) in HEK293 cells. However, unlike the full-size protein, the short isoform of PTTG1 does not affect the MYC gene expression because it lacks the DNA-binding domain, which is needed for its interactions with the MYC promoter. Furthermore, the short form of securin does not influence the expression of MYC transcriptional targets, such as TP53 and IL-8. Thus, we found a novel isoform of securin which is able to activate a more restricted repertoire of genes compared to the full-size protein.
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Sobre autores
D. Demin
Engelhardt Institute of Molecular Biology; Moscow Institute of Physics and Technology
Email: shvarec@yandex.ru
Rússia, Moscow, 119991; Dolgoprydny, Moscow oblast, 141701
A. Bogolyubova
Engelhardt Institute of Molecular Biology; Faculty of Biology
Email: shvarec@yandex.ru
Rússia, Moscow, 119991; Moscow, 119234
D. Zlenko
Faculty of Biology
Email: shvarec@yandex.ru
Rússia, Moscow, 119234
A. Uvarova
Engelhardt Institute of Molecular Biology; Faculty of Biology
Email: shvarec@yandex.ru
Rússia, Moscow, 119991; Moscow, 119234
A. Deikin
Institute of Gene Biology
Email: shvarec@yandex.ru
Rússia, Moscow, 119334
L. Putlyaeva
Engelhardt Institute of Molecular Biology
Email: shvarec@yandex.ru
Rússia, Moscow, 119991
P. Belousov
Engelhardt Institute of Molecular Biology
Email: shvarec@yandex.ru
Rússia, Moscow, 119991
N. Mitkin
Engelhardt Institute of Molecular Biology
Email: shvarec@yandex.ru
Rússia, Moscow, 119991
K. Korneev
Engelhardt Institute of Molecular Biology; Faculty of Biology
Email: shvarec@yandex.ru
Rússia, Moscow, 119991; Moscow, 119234
E. Sviryaeva
Engelhardt Institute of Molecular Biology
Email: shvarec@yandex.ru
Rússia, Moscow, 119991
I. Kulakovskiy
Engelhardt Institute of Molecular Biology; Vavilov Institute of General Genetics
Email: shvarec@yandex.ru
Rússia, Moscow, 119991; Moscow, 119991
K. Tatosyan
Engelhardt Institute of Molecular Biology
Email: shvarec@yandex.ru
Rússia, Moscow, 119991
D. Kuprash
Engelhardt Institute of Molecular Biology; Moscow Institute of Physics and Technology; Faculty of Biology
Email: shvarec@yandex.ru
Rússia, Moscow, 119991; Dolgoprydny, Moscow oblast, 141701; Moscow, 119234
A. Schwartz
Engelhardt Institute of Molecular Biology; Moscow Institute of Physics and Technology
Autor responsável pela correspondência
Email: shvarec@yandex.ru
Rússia, Moscow, 119991; Dolgoprydny, Moscow oblast, 141701
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