Отправить статью по E-mail Hydrogel microchip as a tool for studying exosomes in human serum
- Авторы: Butvilovskaya V.I.1, Tikhonov A.A.1, Savvateeva E.N.1, Ragimov A.A.2, Salimov E.L.2, Voloshin S.A.1, Sidorov D.V.3, Chernichenko M.A.3, Polyakov A.P.3, Filushin M.M.3, Tsybulskaya M.V.1, Rubina A.Y.1
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Учреждения:
- Engelhardt Institute of Molecular Biology
- Sechenov First Moscow State Medical University
- Herzen Moscow Cancer Research Institute
- Выпуск: Том 51, № 5 (2017)
- Страницы: 712-717
- Раздел: Molecular Cell Biology
- URL: https://journals.rcsi.science/0026-8933/article/view/163239
- DOI: https://doi.org/10.1134/S0026893317050053
- ID: 163239
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Аннотация
Exosomes are cell-derived vesicles that are secreted by both normal and cancer cells. Over the last decade, a few studies have revealed that exosomes cross talk and/or influence major tumor-related pathways such as angiogenesis and metastasis involving many cell types within the tumor microenvironment. The protein composition of the membrane of an exosome reflects that of the membrane of the cell of origin. Because of this, tumor-derived exosomes differ from exosomes that are derived from normal cells. The detection of tumor exosomes and analysis of their molecular composition hold promise for diagnosis and prognosis of cancer. Here, we present hydrogel microarrays (biochips), which contain a panel of immobilized antibodies that recognize tetraspanins (CD9, CD63, CD81) and prognostic markers for colorectal cancer (A33, CD147). These biochips make it possible to analyze the surface proteins of either isolated exosomes or exosomes that are present in the serum samples without isolation. These biochips were successfully used to analyze the surface proteins of exosomes from serum that was collected from a colorectal cancer patient and healthy donor. Biochip-guided immunofluorescent analysis of the exosomes has made it possible for us to detect the A33 antigen and CD147 in the serum sample of the colorectal cancer patient with normal levels of CEA and CA19-9.
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Об авторах
V. Butvilovskaya
Engelhardt Institute of Molecular Biology
Автор, ответственный за переписку.
Email: v.butvilovskaya@gmail.com
Россия, Moscow, 119991
A. Tikhonov
Engelhardt Institute of Molecular Biology
Email: v.butvilovskaya@gmail.com
Россия, Moscow, 119991
E. Savvateeva
Engelhardt Institute of Molecular Biology
Email: v.butvilovskaya@gmail.com
Россия, Moscow, 119991
A. Ragimov
Sechenov First Moscow State Medical University
Email: v.butvilovskaya@gmail.com
Россия, Moscow, 119991
E. Salimov
Sechenov First Moscow State Medical University
Email: v.butvilovskaya@gmail.com
Россия, Moscow, 119991
S. Voloshin
Engelhardt Institute of Molecular Biology
Email: v.butvilovskaya@gmail.com
Россия, Moscow, 119991
D. Sidorov
Herzen Moscow Cancer Research Institute
Email: v.butvilovskaya@gmail.com
Россия, Moscow, 125284
M. Chernichenko
Herzen Moscow Cancer Research Institute
Email: v.butvilovskaya@gmail.com
Россия, Moscow, 125284
A. Polyakov
Herzen Moscow Cancer Research Institute
Email: v.butvilovskaya@gmail.com
Россия, Moscow, 125284
M. Filushin
Herzen Moscow Cancer Research Institute
Email: v.butvilovskaya@gmail.com
Россия, Moscow, 125284
M. Tsybulskaya
Engelhardt Institute of Molecular Biology
Email: v.butvilovskaya@gmail.com
Россия, Moscow, 119991
A. Rubina
Engelhardt Institute of Molecular Biology
Email: v.butvilovskaya@gmail.com
Россия, Moscow, 119991
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