电邮这篇文章 Hydrogel microchip as a tool for studying exosomes in human serum
- 作者: Butvilovskaya V.I.1, Tikhonov A.A.1, Savvateeva E.N.1, Ragimov A.A.2, Salimov E.L.2, Voloshin S.A.1, Sidorov D.V.3, Chernichenko M.A.3, Polyakov A.P.3, Filushin M.M.3, Tsybulskaya M.V.1, Rubina A.Y.1
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隶属关系:
- Engelhardt Institute of Molecular Biology
- Sechenov First Moscow State Medical University
- Herzen Moscow Cancer Research Institute
- 期: 卷 51, 编号 5 (2017)
- 页面: 712-717
- 栏目: Molecular Cell Biology
- URL: https://journals.rcsi.science/0026-8933/article/view/163239
- DOI: https://doi.org/10.1134/S0026893317050053
- ID: 163239
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详细
Exosomes are cell-derived vesicles that are secreted by both normal and cancer cells. Over the last decade, a few studies have revealed that exosomes cross talk and/or influence major tumor-related pathways such as angiogenesis and metastasis involving many cell types within the tumor microenvironment. The protein composition of the membrane of an exosome reflects that of the membrane of the cell of origin. Because of this, tumor-derived exosomes differ from exosomes that are derived from normal cells. The detection of tumor exosomes and analysis of their molecular composition hold promise for diagnosis and prognosis of cancer. Here, we present hydrogel microarrays (biochips), which contain a panel of immobilized antibodies that recognize tetraspanins (CD9, CD63, CD81) and prognostic markers for colorectal cancer (A33, CD147). These biochips make it possible to analyze the surface proteins of either isolated exosomes or exosomes that are present in the serum samples without isolation. These biochips were successfully used to analyze the surface proteins of exosomes from serum that was collected from a colorectal cancer patient and healthy donor. Biochip-guided immunofluorescent analysis of the exosomes has made it possible for us to detect the A33 antigen and CD147 in the serum sample of the colorectal cancer patient with normal levels of CEA and CA19-9.
作者简介
V. Butvilovskaya
Engelhardt Institute of Molecular Biology
编辑信件的主要联系方式.
Email: v.butvilovskaya@gmail.com
俄罗斯联邦, Moscow, 119991
A. Tikhonov
Engelhardt Institute of Molecular Biology
Email: v.butvilovskaya@gmail.com
俄罗斯联邦, Moscow, 119991
E. Savvateeva
Engelhardt Institute of Molecular Biology
Email: v.butvilovskaya@gmail.com
俄罗斯联邦, Moscow, 119991
A. Ragimov
Sechenov First Moscow State Medical University
Email: v.butvilovskaya@gmail.com
俄罗斯联邦, Moscow, 119991
E. Salimov
Sechenov First Moscow State Medical University
Email: v.butvilovskaya@gmail.com
俄罗斯联邦, Moscow, 119991
S. Voloshin
Engelhardt Institute of Molecular Biology
Email: v.butvilovskaya@gmail.com
俄罗斯联邦, Moscow, 119991
D. Sidorov
Herzen Moscow Cancer Research Institute
Email: v.butvilovskaya@gmail.com
俄罗斯联邦, Moscow, 125284
M. Chernichenko
Herzen Moscow Cancer Research Institute
Email: v.butvilovskaya@gmail.com
俄罗斯联邦, Moscow, 125284
A. Polyakov
Herzen Moscow Cancer Research Institute
Email: v.butvilovskaya@gmail.com
俄罗斯联邦, Moscow, 125284
M. Filushin
Herzen Moscow Cancer Research Institute
Email: v.butvilovskaya@gmail.com
俄罗斯联邦, Moscow, 125284
M. Tsybulskaya
Engelhardt Institute of Molecular Biology
Email: v.butvilovskaya@gmail.com
俄罗斯联邦, Moscow, 119991
A. Rubina
Engelhardt Institute of Molecular Biology
Email: v.butvilovskaya@gmail.com
俄罗斯联邦, Moscow, 119991
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