Transgenic Expression of chit42 gene from Metarhiziumanisopliae in Trichoderma harzianum Enhances Antagonistic Activity against Botrytis cinerea
- Авторлар: Xia H.1,2, Li Y.Y.1,2, Liu Z.C.1,2, Li Y.Q.1,2, Chen J.1,2,3
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Мекемелер:
- School of Agriculture and Biology, Shanghai Jiaotong University
- The key Laboratory of Urban Agriculture (South), Ministry of Agriculture
- State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University
- Шығарылым: Том 52, № 5 (2018)
- Беттер: 668-675
- Бөлім: Genomics. Transcriptomics
- URL: https://journals.rcsi.science/0026-8933/article/view/163668
- DOI: https://doi.org/10.1134/S002689331805014X
- ID: 163668
Дәйексөз келтіру
Аннотация
Сhitinases expressed by some beneficial fungi are crucial for the biocontrol of phytopathogens. The activity of chitinolytic strains of Trichoderma sp. may be enhanced by increasing the expression of chitinases. We describe the Trichoderma strain Mchit42 which expresses a transgenic chitinase chit42 from Metarhizium anisopliae. Inhibitory effects against plant pathogens were tested. Comparison of WT (T30) and OE (Mchit42) indicated that overexpression of M. anisopliaechit42 did not alter Trichoderma growth, while enhancing the expression of endogenous chitinase, β-1,3-glucanases, and polygalacturonase and increasing the antagonistic activity of Trichoderma against Botrytis cinerea. This work confirmed that the expression of the entomopathogenic fungi-sourced chit42 genes in Trichodermaharzianum enhances the efficiency of Trichoderma biocontrol against targeted pathogens.
Негізгі сөздер
Авторлар туралы
H. Xia
School of Agriculture and Biology, Shanghai Jiaotong University; The key Laboratory of Urban Agriculture (South), Ministry of Agriculture
Email: jiechen59@sjtu.edu.cn
ҚХР, Shanghai, 200240; Shanghai, 200240
Y. Li
School of Agriculture and Biology, Shanghai Jiaotong University; The key Laboratory of Urban Agriculture (South), Ministry of Agriculture
Email: jiechen59@sjtu.edu.cn
ҚХР, Shanghai, 200240; Shanghai, 200240
Z. Liu
School of Agriculture and Biology, Shanghai Jiaotong University; The key Laboratory of Urban Agriculture (South), Ministry of Agriculture
Email: jiechen59@sjtu.edu.cn
ҚХР, Shanghai, 200240; Shanghai, 200240
Y. Li
School of Agriculture and Biology, Shanghai Jiaotong University; The key Laboratory of Urban Agriculture (South), Ministry of Agriculture
Email: jiechen59@sjtu.edu.cn
ҚХР, Shanghai, 200240; Shanghai, 200240
J. Chen
School of Agriculture and Biology, Shanghai Jiaotong University; The key Laboratory of Urban Agriculture (South), Ministry of Agriculture; State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University
Хат алмасуға жауапты Автор.
Email: jiechen59@sjtu.edu.cn
ҚХР, Shanghai, 200240; Shanghai, 200240; Shanghai, 200240
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