Structural and functional analysis of biopolymers and their complexes: Enzymatic synthesis of high-modified DNA
- Autores: Chudinov A.V.1, Kiseleva Y.Y.2, Kuznetsov V.E.1, Shershov V.E.1, Spitsyn M.A.1, Guseinov T.O.1, Lapa S.A.1, Timofeev E.N.1, Archakov A.I.2, Lisitsa A.V.2, Radko S.P.2, Zasedatelev A.S.1
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Afiliações:
- Engelhardt Institute of Molecular Biology
- Orekhovich Institute of Biomedical Chemistry
- Edição: Volume 51, Nº 3 (2017)
- Páginas: 474-482
- Seção: Structural and Functional Analysis of Biopolymers and Their Complexes
- URL: https://journals.rcsi.science/0026-8933/article/view/163114
- DOI: https://doi.org/10.1134/S0026893317030025
- ID: 163114
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Resumo
Here, we describe the synthesis and purification of six deoxyuridine triphosphate derivatives that contain protein-like functional groups and alkene linkers of various lengths. Using KOD XL and Deep Vent polymerases, these derivatives have been incorporated into single-stranded DNA, achieving a high degree of DNA modification. These polymerases are able to utilize highly modified DNA strands as templates for synthesizing unmodified DNA. The synthesized deoxyuridine triphosphate derivatives are promising as substrates for producing modified aptamers to various target proteins using, e.g., the systematic evolution of ligands by exponential enrichment (SELEX) methodology.
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Sobre autores
A. Chudinov
Engelhardt Institute of Molecular Biology
Autor responsável pela correspondência
Email: chud@eimb.ru
Rússia, Moscow, 119991
Y. Kiseleva
Orekhovich Institute of Biomedical Chemistry
Email: chud@eimb.ru
Rússia, Moscow, 119121
V. Kuznetsov
Engelhardt Institute of Molecular Biology
Email: chud@eimb.ru
Rússia, Moscow, 119991
V. Shershov
Engelhardt Institute of Molecular Biology
Email: chud@eimb.ru
Rússia, Moscow, 119991
M. Spitsyn
Engelhardt Institute of Molecular Biology
Email: chud@eimb.ru
Rússia, Moscow, 119991
T. Guseinov
Engelhardt Institute of Molecular Biology
Email: chud@eimb.ru
Rússia, Moscow, 119991
S. Lapa
Engelhardt Institute of Molecular Biology
Email: chud@eimb.ru
Rússia, Moscow, 119991
E. Timofeev
Engelhardt Institute of Molecular Biology
Email: chud@eimb.ru
Rússia, Moscow, 119991
A. Archakov
Orekhovich Institute of Biomedical Chemistry
Email: chud@eimb.ru
Rússia, Moscow, 119121
A. Lisitsa
Orekhovich Institute of Biomedical Chemistry
Email: chud@eimb.ru
Rússia, Moscow, 119121
S. Radko
Orekhovich Institute of Biomedical Chemistry
Email: chud@eimb.ru
Rússia, Moscow, 119121
A. Zasedatelev
Engelhardt Institute of Molecular Biology
Email: chud@eimb.ru
Rússia, Moscow, 119991
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