Structural and functional analysis of biopolymers and their complexes: Enzymatic synthesis of high-modified DNA


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Here, we describe the synthesis and purification of six deoxyuridine triphosphate derivatives that contain protein-like functional groups and alkene linkers of various lengths. Using KOD XL and Deep Vent polymerases, these derivatives have been incorporated into single-stranded DNA, achieving a high degree of DNA modification. These polymerases are able to utilize highly modified DNA strands as templates for synthesizing unmodified DNA. The synthesized deoxyuridine triphosphate derivatives are promising as substrates for producing modified aptamers to various target proteins using, e.g., the systematic evolution of ligands by exponential enrichment (SELEX) methodology.

Sobre autores

A. Chudinov

Engelhardt Institute of Molecular Biology

Autor responsável pela correspondência
Email: chud@eimb.ru
Rússia, Moscow, 119991

Y. Kiseleva

Orekhovich Institute of Biomedical Chemistry

Email: chud@eimb.ru
Rússia, Moscow, 119121

V. Kuznetsov

Engelhardt Institute of Molecular Biology

Email: chud@eimb.ru
Rússia, Moscow, 119991

V. Shershov

Engelhardt Institute of Molecular Biology

Email: chud@eimb.ru
Rússia, Moscow, 119991

M. Spitsyn

Engelhardt Institute of Molecular Biology

Email: chud@eimb.ru
Rússia, Moscow, 119991

T. Guseinov

Engelhardt Institute of Molecular Biology

Email: chud@eimb.ru
Rússia, Moscow, 119991

S. Lapa

Engelhardt Institute of Molecular Biology

Email: chud@eimb.ru
Rússia, Moscow, 119991

E. Timofeev

Engelhardt Institute of Molecular Biology

Email: chud@eimb.ru
Rússia, Moscow, 119991

A. Archakov

Orekhovich Institute of Biomedical Chemistry

Email: chud@eimb.ru
Rússia, Moscow, 119121

A. Lisitsa

Orekhovich Institute of Biomedical Chemistry

Email: chud@eimb.ru
Rússia, Moscow, 119121

S. Radko

Orekhovich Institute of Biomedical Chemistry

Email: chud@eimb.ru
Rússia, Moscow, 119121

A. Zasedatelev

Engelhardt Institute of Molecular Biology

Email: chud@eimb.ru
Rússia, Moscow, 119991

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