Structural properties of capsular and O-specific polysaccharides of Azospirillum brasilense Sp245 under varying cultivation conditions


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Effect of the carbon source in the culture medium and of the growth phase on the composition and structure of the capsular polysaccharides (CPSs) and lipopolysaccharides (LPSs) of the bacterium Azospirillum brasilense Sp245 was studied. Growth with fructose resulted in an increased carbohydrate content in the CPSs, while long-term cultivation resulted in an increased content of phosphorus in both CPSs and LPSs. The LPSs produced on the medium with fructose (regardless of the cultivation duration) and the LPSs of the bacteria grown with sodium malate until the stationary phase were characterized by higher levels of unsaturated fatty acids than the LPSs of the bacteria grown with sodium malate to the late exponential phase. The structures of the polysaccharides from the isolated glycopolymers were established using monosaccharide analysis, including determination of the absolute configurations and 1D and 2D NMR spectroscopy. This study is the first to report that the CPS of A. brasilense Sp245 grown with sodium malate to the end of the exponential phase is structurally identical to the O-polysaccharide from the LPS of this bacterium and that the LPS and CPS of A. brasilense Sp245 grown with fructose contain an additional homoglucan of the following structure: [→3)-α-D-Glcp-(1→]n.

作者简介

S. Yevstigneyeva

Institute of Biochemistry and Physiology of Plants and Microorganisms

编辑信件的主要联系方式.
Email: Stels20295@yandex.ru
俄罗斯联邦, Saratov

E. Sigida

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: Stels20295@yandex.ru
俄罗斯联邦, Saratov

Yu. Fedonenko

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: Stels20295@yandex.ru
俄罗斯联邦, Saratov

S. Konnova

Chernyshevskii National Research Saratov State University

Email: Stels20295@yandex.ru
俄罗斯联邦, Saratov

V. Ignatov

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: Stels20295@yandex.ru
俄罗斯联邦, Saratov

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