Genetic markers for search of rhizobia based on symbiotic genes
- Authors: Akimova E.S.1, Gumenko R.S.1, Vershinina Z.R.1, Baymiev A.K.1, Baymiev A.K.1
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Affiliations:
- Institute of Biochemistry and Genetics
- Issue: Vol 86, No 5 (2017)
- Pages: 640-646
- Section: Experimental Articles
- URL: https://journals.rcsi.science/0026-2617/article/view/163251
- DOI: https://doi.org/10.1134/S0026261717050034
- ID: 163251
Cite item
Abstract
The possible application of rhizobial symbiotic genes as markers for the search and primary identification of rhizobia from temperate-zone legumes was studied. It was shown that conservative sym genes nifH and nifD could be used as markers for rapid search of rhizobia among the analyzed isolates, while more variable genes nifK and nodC could be used for their primary identification. Efficiency of the proposed method was shown in analysis of bacterial isolates obtained from Onobrychis arenaria and Astragalus cicer root nodules.
Keywords
About the authors
E. S. Akimova
Institute of Biochemistry and Genetics
Email: baymiev@anrb.ru
Russian Federation, Ufa
R. S. Gumenko
Institute of Biochemistry and Genetics
Email: baymiev@anrb.ru
Russian Federation, Ufa
Z. R. Vershinina
Institute of Biochemistry and Genetics
Email: baymiev@anrb.ru
Russian Federation, Ufa
Al. Kh. Baymiev
Institute of Biochemistry and Genetics
Email: baymiev@anrb.ru
Russian Federation, Ufa
An. Kh. Baymiev
Institute of Biochemistry and Genetics
Author for correspondence.
Email: baymiev@anrb.ru
Russian Federation, Ufa
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