Genetic markers for search of rhizobia based on symbiotic genes


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Abstract

The possible application of rhizobial symbiotic genes as markers for the search and primary identification of rhizobia from temperate-zone legumes was studied. It was shown that conservative sym genes nifH and nifD could be used as markers for rapid search of rhizobia among the analyzed isolates, while more variable genes nifK and nodC could be used for their primary identification. Efficiency of the proposed method was shown in analysis of bacterial isolates obtained from Onobrychis arenaria and Astragalus cicer root nodules.

About the authors

E. S. Akimova

Institute of Biochemistry and Genetics

Email: baymiev@anrb.ru
Russian Federation, Ufa

R. S. Gumenko

Institute of Biochemistry and Genetics

Email: baymiev@anrb.ru
Russian Federation, Ufa

Z. R. Vershinina

Institute of Biochemistry and Genetics

Email: baymiev@anrb.ru
Russian Federation, Ufa

Al. Kh. Baymiev

Institute of Biochemistry and Genetics

Email: baymiev@anrb.ru
Russian Federation, Ufa

An. Kh. Baymiev

Institute of Biochemistry and Genetics

Author for correspondence.
Email: baymiev@anrb.ru
Russian Federation, Ufa

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