Differentiation of Juniperus deltoidеs R.P. Аdams in the Crimean-Caucasian Region According to the Variability of Microsatellite DNA Markers

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Abstract

*e-mail: hantemirova@ipae.uran.ru

The structure of genetic variability of the prickly juniper Juniperus deltoides R.P. Adams (family Cupressaceae), an important component of Mediterranean arid and semi-arid ecosystems was studied. We used for the first time 5 nuclear microsatellite loci developed for another juniper species J. cedrus Webb & Berthel. to genotype samples from 5 populations of J. deltoides located at the northeastern limit of the range in Eurasia (Western Crimea, Transcaucasia) and one Balkan population (Bulgaria). J. deltoides is characterized by an average level of genetic variability (He varies from 0.428 to 0.602) with the lowest values in the Crimean populations. Phylogenetic analyses revealed three genetic groups: Western Crimea, Transcaucasia (Krasnodar Territory) and Bulgarian population. Application of AMOVA to these groups showed statistically significant differentiation (9.9% of total variability, P < 0.001). The first two groups correspond to the previously identified Asian group of J. deltoides, and the third group corresponds to the Balkan group. The differentiation of the Crimean populations from geographically close Caucasian populations is shown by us for the first time.

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About the authors

E. V. Hantemirova

Institute of Plant and Animal Ecology, Ural Division of the Russian Academy of Sciences

Author for correspondence.
Email: hantemirova@ipae.uran.ru
Russian Federation, Ekaterinburg, 620144

Tz. Radoukova

University of Plovdiv “Paisii Hilendarski”

Email: hantemirova@ipae.uran.ru

Department of Botany and Biological Education

Bulgaria, Plovdiv, 4000

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Supplementary files

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2. Fig. 1. Ranges of Juniperus deltoides (red) and J. oxycedrus (blue) according to R.P. Adams [3].

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3. Fig. 2. Outlines of the study area with the location of the studied populations of J. deltoides, distribution of genetic groups, and graphs with SSR polymorphism estimates. Population numbers and geographic coordinates are explained in Table 1. a – distribution of genetic groups of J. deltoidеs based on SSR marker polymorphism. The color of the icons corresponds to the SAMOVA and PCoA groups. b – ordination of J. deltoides populations by the principal coordinate method (PCoA) based on their genetic distances; Roman numerals indicate groups according to SAMOVA. c – UPGMA dendrogram of J. deltoides populations constructed on the basis of their genetic distances in the NTSYSpc 2.1 program. d – results of the STRUCTURE analysis.

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