Comparative Analysis of the HAP2/GCS1, GEX2 Genes Expression in Maize Lines of Saratov Selection

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Abstract

The haploinduction phenomenon have a scientific and practical aspects for development the effective haploid-inducing maize line and diploid homozygous lines creation. The article discusses the relationship between the maize haploid–induction and the violation of the gamete interactions, in particular for GEX2-HAP2/GCS1-mediated adhesion and fusion of gamete membranes in Zarodishevii Marker Saratovskii Purpurnii (ZMSP) maize lines. Using real-time RT-PCR method, it was found that the GEX2, HAP2/GCS1 genes are expressed in sperm and ovules, but the maize haploid-inducing ability does not correlate with their expression. It was shown that the ZMSP haploid-inducing line have 27 SNP, one 9-bp insertion, 2-bp SNP and the corresponding 10 amino acid substitutions with two insertions in the GEX2 protein in comparing with reference B73 line. It was found that HAP2/GCS1, as a conservative protein for membrane fusion has only one amino acid substitution in ZMSP in comparing with B73 maize and Brown Marker maize lines.

About the authors

E. M. Moiseeva

Institute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Federal
Research Center, Saratov Scientific Centre of the Russian Academy of Sciences

Email: chumakov_m@ibppm.ru
Russia, 410049, Saratov

Yu. S. Gusev

Institute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Federal
Research Center, Saratov Scientific Centre of the Russian Academy of Sciences; Chernyshevsky Saratov National Research State University

Email: chumakov_m@ibppm.ru
Russia, 410049, Saratov; Russia, 410012, Saratov

O. V. Gutorova

Chernyshevsky Saratov National Research State University

Email: chumakov_m@ibppm.ru
Russia, 410012, Saratov

M. I. Chumakov

Institute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Federal
Research Center, Saratov Scientific Centre of the Russian Academy of Sciences

Author for correspondence.
Email: chumakov_m@ibppm.ru
Russia, 410049, Saratov

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Copyright (c) 2023 Е.М. Моисеева, Ю.С. Гусев, О.В. Гуторова, М.И. Чумаков

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