Advances in Chemical PhysicsAdvances in Chemical PhysicsФизиология растений0015-33033034-624XThe Russian Academy of Sciences13021910.31857/S0015330322600693IBHUOHЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleEarly Blight Resistance of Transgenic Potato Plants Expressingthe ProSmAMP1 Gene for Antimicrobial Peptides under the Control of a Light-Inducible Cab PromoterУстойчивость к альтернариозу трансгенных растений картофеля, экспрессирующих ген антимикробных пептидов ProSmAMP1 под контролем светоиндуцибельного промотораBeliaevD. V.БеляевД. В.
Russian Federation
bdv@ippras.ruYourievaN. O.ЮрьеваН. О.
Russian Federation
bdv@ippras.ruTereshonokD. V.ТерешонокД. В.
Russian Federation
bdv@ippras.ruDerevyaginaM. K.ДеревягинаМ. К.
Russian Federation
bdv@ippras.ruMeleshinA. A.МелешинА. А.
Russian Federation
bdv@ippras.ruTimiryazev Institute of Plant Physiology, Russian Academy of SciencesФедеральное государственное бюджетное учреждение науки Институт физиологии растений им. К.А. Тимирязева Российской академии наукRussian Potato Research CentreФедеральное государственное бюджетное научное учреждение Федеральный исследовательский центр картофеля имени А.Г. Лорха0105202370331932621082023Copyright ©; 2023, Д.В. Беляев, Н.О. Юрьева, Д.В. Терешонок, М.К. Деревягина, А.А. МелешинCopyright ©; 2023, Д.В. Беляев, Н.О. Юрьева, Д.В. Терешонок, М.К. Деревягина, А.А. Мелешин2023Д.В. Беляев, Н.О. Юрьева, Д.В. Терешонок, М.К. Деревягина, А.А. МелешинД.В. Беляев, Н.О. Юрьева, Д.В. Терешонок, М.К. Деревягина, А.А. Мелешинhttps://journals.rcsi.science/0015-3303/article/view/130219

The genome of Stellaria media contains a gene family for hevein-like antimicrobial peptides, some of which are known to encode two peptides released from the translation product as a result of post-translational proteolysis. These peptides have been shown to inhibit the growth of bacteria and fungi, including potato pathogens Alternaria solani and Alternaria alternata. One of these genes, ProSmAMP1, was introduced into the potato genome under the control of the light-inducible promoter of Cab gene from common wheat. The resulting transgenic lines expressed ProSmAMP1 mRNA during several vegetative passages, and their resistance to early blight was assessed by several indicators of detached leaf infection, with plants having the highest expression of the transgene also showing the highest resistance.

Геном растения звездчатка Stellaria media содержит семейство генов гевеин-подобных антимикробных пептидов, про некоторые из которых известно, что они кодируют два пептида, высвобождающиеся из продукта трансляции в результате пост-трансляционного протеолиза. Ранее было показано, что данные пептиды подавляют рост бактерий и грибов, в том числе, патогенов картофеля Alternaria solani и Alternaria alternata. В данной работе один из таких генов, ProSmAMP1, был введен в геном картофеля под контролем светоиндуцибельного промотора гена Cab мягкой пшеницы. Полученные трансгенные линии экспрессировали мРНК ProSmAMP1 в течение нескольких вегетативных пассажей и их устойчивость к альтернариозу была оценена по нескольким показателям заражения отделенных листьев, причем растения с наибольшей экспрессией трансгена продемонстрировали также наибольшую устойчивость.

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