Advances in Chemical PhysicsAdvances in Chemical PhysicsФизиология растений0015-33033034-624XThe Russian Academy of Sciences13021810.31857/S0015330322600619IASUAIЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleEffect of Photoperiod Duration on Efficiency of Low-Temperature Hardening of Arabidopsis thaliana Heynh. (L.)Влияние продолжительности фотопериода на эффективность низкотемпературного закаливания Arabidopsis thaliana Heynh. (L.)PopovValery N.ПоповВ. Н.
Russian Federation
vnpopov@mail.ruDeryabinAlexander N.ДерябинА. Н.
Russian Federation
vnpopov@mail.ruTimiryazev Institute of Plant Physiology, Russian Academy of SciencesФедеральное государственное бюджетное учреждение науки Институт физиологии растений им. К.А. Тимирязева Российской академии наук0105202370331131821082023Copyright ©; 2023, В.Н. Попов, А.Н. ДерябинCopyright ©; 2023, В.Н. Попов, А.Н. Дерябин2023В.Н. Попов, А.Н. ДерябинВ.Н. Попов, А.Н. Дерябинhttps://journals.rcsi.science/0015-3303/article/view/130218

The effect of photoperiod duration on efficiency of low-temperature hardening was investigated in Arabidopsis thaliana (L.) Heynh. plants, ecotype Col-0. Six-week-old plants were exposed to cold acclimation at a temperature of 2°С during 1‒5 days at photoperiods of 0, 8, and 16 h (illuminance of 200 µmol/(m2 s)). According to survival data and leakage of electrolytes after test freezing (–6°C, 24 h), the plants exposed to cold acclimation in the dark did not show frost resistance. The plants hardened in the light (irrespective of the length of photoperiod) considerably improved their frost resistance by the end of the cold-acclimation period. Net photosynthesis/dark respiration ratio in these plants was almost two times greater than in control material (without hardening). The plants exposed to a 16-h-long photoperiod surpassed the type of treatment with 8-h-long illumination both in the highest levels of accumulation of sugars (by almost 40%) and in the rate of reaching these levels in daily dynamics of hardening. It was shown that MDA content transiently rose during the first 24 h of hardening in the light and did not change in the dark, which may point to a signal role of lipid peroxidation products upon cold acclimation. It was discovered that the photoperiod duration affected the formation rate of frost resistance in A. thaliana plants. A more prolonged operation of A. thaliana’s photosynthetic apparatus at 16-h-long photoperiod considerably accelerated the accumulation of sugars upon cold acclimation and, therefore, hastened development of frost resistance as compared with an 8-h-long photoperiod. It was concluded that rapid formation of frost resistance in A. thaliana requires a combination of low above-zero temperature and 16-h-long photoperiod.

Исследовали влияние продолжительности фотопериода на эффективность низкотемпературного закаливания растений Arabidopsis thaliana (L.) Heynh., экотип Col-0. Закаливание 6-недельных растений проводили при температуре 2°С в течение 1‒5 сут., используя фотопериоды 0, 8 и 16 ч (освещенность 200 мкмоль/(м2 с)). Показано, что растения, подвергнутые закаливанию в темноте, не были устойчивы к морозу, согласно данным по выживаемости и выходу электролитов после тестирующего промораживания (–6°C, 1 сут.). Растения, закаленные на свету (независимо от фотопериода), к концу периода закаливания значительно повысили свою морозоустойчивость. Отношение видимый фотосинтез/темновое дыхание у этих растений было почти в два раза выше, чем в контроле (без закаливания). Вариант с 16-часовым фотопериодом превосходил вариант с 8-часовым освещением, как по максимальным уровням накопления сахаров (почти на 40%), так и по скорости достижения этих уровней в посуточной динамике закаливания. Показано транзиторное увеличение содержания МДА в первые сутки закаливания на свету и отсутствие изменений в содержании МДА при закаливании в темноте, что может свидетельствовать о сигнальной функции продуктов перекисного окисления липидов при закаливании. Установлено влияние продолжительности фотопериода на скорость формирования устойчивости растений A. thaliana к морозу. Более длительная работа фотосинтетического аппарата A. thaliana при 16 ч фотопериоде значительно повышала скорость накопления сахаров при закаливании и, соответственно, скорость формирования устойчивости к морозу по сравнению с 8 ч фотопериодом. Заключено, что для быстрого формирования повышенной устойчивости A. thaliana к морозу требуется сочетание низкой положительной температуры и 16-часового фотопериода.

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