Advances in Chemical PhysicsAdvances in Chemical PhysicsФизиология растений0015-33033034-624XThe Russian Academy of Sciences13021310.31857/S0015330322600796IBVUFZЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleThe Role of SnRK1 Kinase in the Response of the Photosynthetic Machinery to Salinity StressРоль киназы SnRK1 в ответе фотосинтетического аппарата на солевой стрессMurtuzovaAlexandra V.МуртузоваА. В.
Russian Federation
ETutereva@binran.ruTyuterevaElena V.ТютереваЕ. В.
Russian Federation
ETutereva@binran.ruVoitsekhovskajaOlga V.ВойцеховскаяО. В.
Russian Federation
ETutereva@binran.ruLaboratory of Molecular and Ecological Physiology, Komarov Botanical Institute, Russian Academy of SciencesФедеральное государственное бюджетное учреждение науки “Ботанический институт имени В.Л. Комарова Российской академии наук”0105202370327929221082023Copyright ©; 2023, А.В. Муртузова, Е.В. Тютерева, О.В. ВойцеховскаяCopyright ©; 2023, А.В. Муртузова, Е.В. Тютерева, О.В. Войцеховская2023А.В. Муртузова, Е.В. Тютерева, О.В. ВойцеховскаяА.В. Муртузова, Е.В. Тютерева, О.В. Войцеховскаяhttps://journals.rcsi.science/0015-3303/article/view/130213

In plants, SnRK1 (Sucrose non-fermenting-Related protein Kinase 1) is one of the major activators of catabolic processes, including autophagy, during stress responses. SnRK1 generally acts as a sensor of the energy status of the cell. Photosynthesis is by far the largest energy-supplying process in green plant cells exposed to light; thus, SnRK1 might participate in its regulation. In leaves of Arabidopsis lines with different levels of the catalytic subunit of SnRK1, KIN10, quantum yields of photosystems and of non-photochemical quenching, formation of the transthylakoid proton motive force, and contents of ATP in seedlings were compared under optimal conditions and under salinity stress. We detected specific changes in the photochemical activity of the chloroplasts that were assigned to constant activation of SnRK1 in two lines with constitutive overexpression of KIN10, both under control conditions and under salinity stress. Furthermore, the inhibition of the SnRK1 activity by means of RNA interference in Arabidopsis led to a lack of response to salinity at the level of chloroplast photochemistry.

Киназа SnRK1 (Sucrose non-fermenting-Related protein Kinase 1) растений регулирует активацию катаболических процессов, включая автофагию, в ходе стрессовых ответов. SnRK1 часто рассматривают как сенсор энергетического статуса клетки. Фотосинтез является крупнейшим процессом, поставляющим энергию зеленым клеткам растений на свету, и можно полагать, что SnRK1 является одним из его регуляторов. В листьях линий Arabidopsis, различающихся уровнем экспрессии гена KIN10, кодирующего каталитическую субъединицу SnRK1, сравнивали квантовый выход фотосистем и нефотохимического тушения флуоресценции, формирование электрохимического градиента протонов на тилакоидных мембранах, а также содержание АТФ в проростках в оптимальных условиях и при воздействии солевого стресса. В результате проведенного исследования нами показаны изменения фотохимической активности хлоропластов, ассоциированные с конститутивной активацией SnRK1 киназы в двух линиях, сверхэкспрессирующих каталитическую субъединицу KIN10. Обнаруженные особенности световых реакций фотосинтеза сохранялись в этих линиях и при благоприятных условиях роста, и при адаптации к солевому стрессу. Подавление функции SnRK1 киназы методом замалчивания РНК-интерференцией в линиях арабидопсиса, напротив, приводило к отсутствию выраженного ответа на солевой стресс на уровне фотохимической активности хлоропластов.

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