Advances in Chemical PhysicsAdvances in Chemical PhysicsФизиология растений0015-33033034-624XThe Russian Academy of Sciences13021110.31857/S0015330322600735IBRXKHЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleCharacteristics of a New Halotolerant Arctic Strain of Carotenogenic Microalga Halochlorella rubescens NAMSU SBB-20Характеристика нового галотолерантного арктического штамма каротиногенной микроводоросли Halochlorella rubescens NAMSU SBB-20ZaitsevaA. A.ЗайцеваА. А.
Russian Federation
annakublanovskaya@gmail.comBakharevaD. A.БахареваД. А.
Russian Federation
annakublanovskaya@gmail.comZaitsevP. A.ЗайцевП. А.
Russian Federation
annakublanovskaya@gmail.comLobakovaE. S.ЛобаковаЕ. С.
Russian Federation
annakublanovskaya@gmail.comMoscow State UniversityФедеральное государственное бюджетное образовательное учреждение высшего образования “Московский государственный университет имени М.В. Ломоносова”0105202370326927821082023Copyright ©; 2023, А.А. Зайцева, Д.А. Бахарева, П.А. Зайцев, Е.С. ЛобаковаCopyright ©; 2023, А.А. Зайцева, Д.А. Бахарева, П.А. Зайцев, Е.С. Лобакова2023А.А. Зайцева, Д.А. Бахарева, П.А. Зайцев, Е.С. ЛобаковаА.А. Зайцева, Д.А. Бахарева, П.А. Зайцев, Е.С. Лобаковаhttps://journals.rcsi.science/0015-3303/article/view/130211

Green microalgae capable of accumulating secondary carotenoids are the most important objects of biotechnology, and the search for new strains with unique properties, in particular, those adapted to growth at low temperatures and high salinity in the environment, is an urgent task. The NAMSU SBB-20 microalga strain was isolated from an algal-bacterial biofilm found on the coast of the White Sea in the littoral zone of the Solovetsky Archipelago. Identification of the strain showed its belonging to the species Halochlorella rubescens P.J.L.Dangeard. The species H. rubescens was first described for the White Sea. Under conditions of high light intensity, ultrastructural changes in cells are shown, among which destruction of the photosynthetic apparatus and the formation of cytoplasmic and chloroplast lipid inclusions are noted. It was shown that the culture of the NAMSU SBB-20 strain is capable of acquiring an orange color under unfavorable growth conditions. An assessment was made of the effect of the composition of the medium and the intensity of illumination on the pigment composition of the algae. The highest absolute values of the accumulation of carotenoids were noted during cultivation in light with an intensity of 150 μmol PAR quanta/m2/s on BG-11 media containing no source of phosphorus (15.66 ± 0.18 mg/L) or nitrogen (15.95 ± 0.56 mg/L). The described strain has a biotechnological potential due to the initial halotolerance and the accumulation of high values of secondary carotenoids in the biomass.

Зеленые микроводоросли, способные к накоплению вторичных каротиноидов, являются важнейшими объектами биотехнологии, а поиск новых штаммов с уникальными свойствами, в частности, приспособленных к росту при низких температурах и повышенной солености в среде – актуальной задачей. Штамм микроводоросли NAMSU SBB-20 выделен из альго-бактериальной биопленки, обнаруженной на побережье Белого моря в зоне литорали на Соловецком архипелаге. Идентификация штамма показала его принадлежность к виду Halochlorella rubescens P.J.L.Dangeard. Вид H. rubescens впервые описан для акватории Белого моря. В условиях высокой интенсивности света показаны ультраструктурные изменения клеток, среди которых отмечены деструкция фотосинтетического аппарата, формирование цитоплазматических и хлоропластных липидных включений. Показано, что в неблагоприятных для роста условиях культура штамма NAMSU SBB-20 способна приобретать оранжевую окраску. Проведена оценка влияния состава среды и интенсивности освещения на пигментный состав водоросли. Наибольшие абсолютные значения накопления каротиноидов отмечены при культивировании на свету интенсивностью 150 мкмоль квантов ФАР/м2/с на средах BG-11, не содержащих источника фосфора (15.66 ± 0.18 мг/л) или азота (15.95 ± 0.56 мг/л). Описанный штамм обладает биотехнологическим потенциалом ввиду изначальной галотолерантности и накопления высоких значений вторичных каротиноидов в биомассе.

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