Advances in Chemical PhysicsAdvances in Chemical PhysicsФизиология растений0015-33033034-624XThe Russian Academy of Sciences13020410.31857/S0015330322600747IBTJGRЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleCoexpression of Structural and Regulatory Genes of the Flavonoid Pathway Reveals the Characteristics of Anthocyanin Biosynthesis in Eggplant Organs (Solanum melongena L.)Коэкспрессия структурных и регуляторных генов флавоноидного пути выявляет особенности биосинтеза антоцианов в органах баклажана (Solanum melongena L.)FilyushinM. A.ФилюшинМ. А.
Russian Federation
michel7753@mail.ruShchennikovaA. V.ЩенниковаА. В.
Russian Federation
michel7753@mail.ruKochievaE. Z.КочиеваЕ. З.
Russian Federation
michel7753@mail.ruFederal Research Center Fundamentals of Biotechnology, Russian Academy of SciencesФедеральный исследовательский центр “Фундаментальные основы биотехнологии” Российской академии наук0105202370324125021082023Copyright ©; 2023, М.А. Филюшин, А.В. Щенникова, Е.З. КочиеваCopyright ©; 2023, М.А. Филюшин, А.В. Щенникова, Е.З. Кочиева2023М.А. Филюшин, А.В. Щенникова, Е.З. КочиеваМ.А. Филюшин, А.В. Щенникова, Е.З. Кочиеваhttps://journals.rcsi.science/0015-3303/article/view/130204

Eggplant (Solanum melongena L.) is an economically important vegetable crop whose purple-colored fruits are enriched with anthocyanidins. In this work in the eggplant genome, homologues of the main known structural (CHS1, CHS2, CHI, F3H, F3´5´H, DFR, ANS, and UFGT) and regulatory (TT8, GL3, bHLH137, bHLH143, MYB1, MYB2, and MYB75) anthocyanin biosynthesis genes, as well as anthocyanidin transporter gene (GSTF12), were identified. The expression of these genes was characterized in comparisonwith the content of the total anthocyanins and the color of the leaf, flower petals, and fruit peel. It was shown that the gene expression pattern corresponds to the color and the presence of anthocyanins in the tissue, and also indicates the presence of organ-specific characteristics of the regulation of transcription of genes encoding transcription factors of the MBW complex. The results of correlation analysis confirm the involvement of SmbHLH137, SmTT8, SmMYB2, and SmMYB75 genes in the regulation of the expression of structural genes in flower petals and SmGL3, SmTT8, and SmMYB1 in fruit peel.

Баклажан (Solanum melongena L.) является экономически значимой овощной культурой, фиолетово-окрашенные плоды которого обогащены антоцианидинами. В данной работе в геноме баклажана были идентифицированы гомологи основных известных структурных (CHS1, CHS2, CHI, F3H, F3´5´H, DFR, ANS и UFGT) и регуляторных (TT8, GL3, bHLH137, bHLH143, MYB1, MYB2 и MYB75) генов биосинтеза антоцианов, а также гена транспортера антоцианидинов (GSTF12). Охарактеризована экспрессия данных генов в сопоставлении с содержанием суммы антоцианов и окраской листа, лепестков цветка и кожицы плода. Показано, что профиль экспрессии генов соответствует окраске и присутствию антоцианов в ткани, а также указывает на существование органоспецифичных особенностей регуляции транскрипции генов, кодирующих транскрипционные факторы комплекса MBW. Результаты корреляционного анализа подтверждают участие генов SmbHLH137, SmTT8, SmMYB2 и SmMYB75 в регуляции экспрессии структурных генов в лепестках цветка, а SmGL3, SmTT8 и SmMYB1 – в кожице плода.

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