Tissue-Specific Peculiarities of Vibration-Induced Hypoxia in Rabbit Liver and Kidney

详细

Activity of the energy production systems in rabbit liver and kidney under conditions of unfavorable vibration exposure was studied by the polarography method using a galvanic-type closed oxygen sensor. The rate of oxidation of endogenous substrates by mitochondria was determined by the tissue and was 5.2±0.6 and 8.13±1.4 (ng-atom O)×min^—1×mg^—1 protein for liver and kidney of intact animals, respectively. After 21 vibration sessions against the background of inhibition of NAD-dependent substrate oxidation in liver mitochondria, the rate metabolism of exogenous succinic acid increased by 44% and then decreased with prolongation of the effect, which indicated impaired function of the respiratory chain. Similar fluctuations of the parameters were revealed in kidney mitochondria, though their amplitude was lower. The study of bioenergetic mechanisms of hypoxia in various tissues makes it possible to determine the targets for the pharmacological action of antihypoxic drugs.