A Study of the Cytotoxic Effect of Microcapsules and Their Constituent Polymers on Macrophages and Tumor Cells
- 作者: Naumov A.1, Dubrovskii A.1, Musin E.1, Kim A.1, Potselueva M.1,2, Tikhonenko S.1
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隶属关系:
- Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
- Pushchino State Institute of Natural Sciences, Russian Academy of Sciences
- 期: 卷 166, 编号 1 (2018)
- 页面: 69-74
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240836
- DOI: https://doi.org/10.1007/s10517-018-4291-7
- ID: 240836
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详细
We studied the effect of different concentrations of polyelectrolytes poly(allylamine hydrochloride) (PAH) and polystyrene sulfonate (PSS) as well as the effects of microcapsules coated with these polymers on survival of Ehrlich ascites carcinoma cells and mouse peritoneal macrophages and on ROS production by phagocytes. PAH reduced viability of Ehrlich ascites carcinoma in a concentration-dependent manner (LD50=12-15 μg/ml). This effect was presumably determined by its ability to bind phosphates, thereby depleting the culture medium. At the same time, PAH did not affect the viability of macrophages. PSS produced no cytotoxic effect on the examined cells. Polyelectrolyte capsules with the shell architectonics (PAH/PSS)3 and (PAH/PSS)3PAH in the examined concentration range had no effect on the viability of macrophages and tumor cells. PAH microcapsules with positively charged surface much more rapidly and more intensively activated macrophages. The chemiluminescence response directly depended on the amount of capsules in the solution.
作者简介
A. Naumov
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Email: tikhonenkosa@gmail.com
俄罗斯联邦, Pushchino, Moscow Region
A. Dubrovskii
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Email: tikhonenkosa@gmail.com
俄罗斯联邦, Pushchino, Moscow Region
E. Musin
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Email: tikhonenkosa@gmail.com
俄罗斯联邦, Pushchino, Moscow Region
A. Kim
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Email: tikhonenkosa@gmail.com
俄罗斯联邦, Pushchino, Moscow Region
M. Potselueva
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences; Pushchino State Institute of Natural Sciences, Russian Academy of Sciences
Email: tikhonenkosa@gmail.com
俄罗斯联邦, Pushchino, Moscow Region; Pushchino, Moscow Region
S. Tikhonenko
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: tikhonenkosa@gmail.com
俄罗斯联邦, Pushchino, Moscow Region