Generation of Highly Specific Proteolytic Biocatalysts by Screening Technologies
- 作者: Bobik T.1, Kostin N.1, Knorre V.1, Gabibov A.1, Smirnov I.1
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隶属关系:
- Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Organic Biochemistry, Russian Academy of Sciences
- 期: 卷 165, 编号 3 (2018)
- 页面: 399-402
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240354
- DOI: https://doi.org/10.1007/s10517-018-4179-6
- ID: 240354
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详细
We propose a yeast display-based system for screening of proteolytic enzyme libraries that utilizes substrate protein adsorbed on the yeast cell surface and containing a desired cleavage sequence. Specific cleavage of the substrate protein releases its biotin-binding center. The cells carrying the target proteinase can be selected by cytofluorometry due to interaction with biotinylated fluorescent protein. Using human enterokinase light chain as the model proteinase we showed that the proposed screening system highly effectively selects the proteolytic enzymes with preset specificity.
作者简介
T. Bobik
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Organic Biochemistry, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
N. Kostin
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Organic Biochemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
V. Knorre
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Organic Biochemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
A. Gabibov
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Organic Biochemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
I. Smirnov
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Organic Biochemistry, Russian Academy of Sciences
Email: bobik_tanya@mail.ru
俄罗斯联邦, Moscow
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