Comparative Study of Fusogenic Activity of H1 and H5 Subtypes Influenza Virus Hemagglutinins
- 作者: Kononova A.1, Cheresiz S.1, Chechushkov A.2, Razumova Y.1, Pokrovskii A.1
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隶属关系:
- Novosibirsk National Research State University
- Research Institute of Experimental and Clinical Medicine
- 期: 卷 164, 编号 1 (2017)
- 页面: 85-89
- 栏目: Virology
- URL: https://journals.rcsi.science/0007-4888/article/view/239383
- DOI: https://doi.org/10.1007/s10517-017-3930-8
- ID: 239383
如何引用文章
详细
Influenza virus hemagglutinins are surface proteins responsible for fusion of the viral and cellular membranes. Their capacity to mediate membrane fusion (fusogenic activity) is studied by various methods, including the syncytium formation and pseudovirus transduction methods. We constructed plasmids coding for genes of three H1 and one H5 hemagglutinins and compared their fusogenic activities. Hemagglutinin capacity to induce syncytium formation did not always correlate with the transduction activity of the respective pseudoviruses. Hemagglutinin H5 exhibited high fusogenic activity in studies by both methods, however, two of the studied H1 hemagglutinins induced the formation of syncytia, but did not mediate pseudovirus transduction. This could be due to different capsid sizes of influenza virus and vesicular stomatitis virus, which determines their different permeability through the fusion pore.
作者简介
A. Kononova
Novosibirsk National Research State University
编辑信件的主要联系方式.
Email: alenkononova@yandex.ru
俄罗斯联邦, Novosibirsk
S. Cheresiz
Novosibirsk National Research State University
Email: alenkononova@yandex.ru
俄罗斯联邦, Novosibirsk
A. Chechushkov
Research Institute of Experimental and Clinical Medicine
Email: alenkononova@yandex.ru
俄罗斯联邦, Novosibirsk
Yu. Razumova
Novosibirsk National Research State University
Email: alenkononova@yandex.ru
俄罗斯联邦, Novosibirsk
A. Pokrovskii
Novosibirsk National Research State University
Email: alenkononova@yandex.ru
俄罗斯联邦, Novosibirsk