Studying the Toxic Effects of Some Biologically Active Peptides on the Model of Mouse Embryonic Stem Cells
- 作者: Kobylyanskii A.1, Zolotarev Y.1, Andreeva L.1, Grivennikov I.1, Myasoedov N.1
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隶属关系:
- Institute of Molecular Genetics, Russian Academy of Sciences
- 期: 卷 163, 编号 6 (2017)
- 页面: 731-736
- 栏目: Pharmacology and Toxicology
- URL: https://journals.rcsi.science/0007-4888/article/view/239221
- DOI: https://doi.org/10.1007/s10517-017-3891-y
- ID: 239221
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详细
We studied the effects of peptide drugs (HLDF-6, PGP, RPGP, and PGLP) and peptide pharmaceutical products (Semax, Selank, and thyroliberin) on proliferation and survival of mouse embryonic stem cells and their derivatives. Differentiation of mouse embryonic stem cells into neuronal precursors was evaluated. PGP and PGLP in concentrations of 10 and 0.1 μM, respectively, had little, but significant inhibitory effect on proliferative activity of cells. These peptides in concentrations of 10 and 0.1 μM, respectively, and Semax (10 and 0.1 μM) significantly increased the survival rate of mouse embryonic stem cells (serum deprivation). Moreover, study peptides had little effect on the formation of neuronal precursors from mouse embryonic stem cells. HLDF-6, Selank, and thyroliberin produced an insignificant effect on the differentiation of these cells into mature neurons. Analysis of differentiation of embryonic stem cells into GABA+ neurons showed that Selank, thyroliberin (100 μM), and NGF (100 ng/ml) decrease the ratio of these cells by 61, 58, and 87%, respectively, in comparison with the control. Our results indicate that these peptide compounds do not produce toxic effect during the embryonic and fetal period of life.
作者简介
A. Kobylyanskii
Institute of Molecular Genetics, Russian Academy of Sciences
编辑信件的主要联系方式.
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俄罗斯联邦, Moscow
Yu. Zolotarev
Institute of Molecular Genetics, Russian Academy of Sciences
Email: testers@yandex.ru
俄罗斯联邦, Moscow
L. Andreeva
Institute of Molecular Genetics, Russian Academy of Sciences
Email: testers@yandex.ru
俄罗斯联邦, Moscow
I. Grivennikov
Institute of Molecular Genetics, Russian Academy of Sciences
Email: testers@yandex.ru
俄罗斯联邦, Moscow
N. Myasoedov
Institute of Molecular Genetics, Russian Academy of Sciences
Email: testers@yandex.ru
俄罗斯联邦, Moscow