Isolation of Induced Pluripotent Cells from Stromal Liver Cells of Patients with Alcoholic Cirrhosis
- 作者: Kholodenko I.1, Kholodenko R.2, Manukyan G.3, Lupatov A.1, Yarygin K.1
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隶属关系:
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- B. V. Petrovsky Russian Research Center of Surgery, Russian Academy of Medical Sciences
- 期: 卷 163, 编号 4 (2017)
- 页面: 535-541
- 栏目: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239076
- DOI: https://doi.org/10.1007/s10517-017-3845-4
- ID: 239076
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详细
Stromal liver cells obtained from liver biopsy specimens of a patient with alcoholic cirrhosis can proliferate for a long time in culture passing more than 30 passages. In the course of culturing from early to late passages, acceleration of cell proliferation, decrease of the expression of some markers, and loss of hepatogenic differentiation potential were observed. On passage 30, induced pluripotent stem cells were obtained from these cells and comparative analysis of adipogenic and hepatic differentiation potencies of these cells and original liver stromal cells was performed. Induced pluripotent stem cells differentiated into both directions more efficiently and more rapidly than initial cells. Under conditions of hepatic differentiation, liver stromal cells started to express markers of definitive endoderm, but not markers of immature/mature hepatocytes, whereas induced pluripotent stem cells consistently expressed markers of definitive endoderm, immature/mature hepatocytes.
作者简介
I. Kholodenko
V. N. Orekhovich Research Institute of Biomedical Chemistry
编辑信件的主要联系方式.
Email: irkhol@yandex.ru
俄罗斯联邦, Moscow
R. Kholodenko
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: irkhol@yandex.ru
俄罗斯联邦, Moscow
G. Manukyan
B. V. Petrovsky Russian Research Center of Surgery, Russian Academy of Medical Sciences
Email: irkhol@yandex.ru
俄罗斯联邦, Moscow
A. Lupatov
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: irkhol@yandex.ru
俄罗斯联邦, Moscow
K. Yarygin
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: irkhol@yandex.ru
俄罗斯联邦, Moscow