Sodium Dodecyl Sulfate Cytotoxicity towards HaCaT Keratinocytes: Comparative Analysis of Methods for Evaluation of Cell Viability
- 作者: Rusanov A.1, Luzgina N.2, Lisitsa A.2
-
隶属关系:
- Perspective Company
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- 期: 卷 163, 编号 2 (2017)
- 页面: 284-288
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238825
- DOI: https://doi.org/10.1007/s10517-017-3785-z
- ID: 238825
如何引用文章
详细
Viability of keratinocytes of HaCaT immortalized line incubated with sodium dodecyl sulfate for 3 min, 1 and 48 h, was studied by light microscopy, MTT test, and neutral red absorption test. The IC50 values were determined for each of the studied lengths of exposure. HaCaT cells exhibited a dose-dependent decrease of viability under the effect of sodium dodecyl sulfate, proportional to the length of exposure. The values measured by different methods (MTT test and neutral red absorption test) varied, the differences were determined by the duration of exposure to sodium dodecyl sulfate. The dispersion of values for 1 and 48 h exposure, obtained by MTT method, was greater than of the values obtained by neutral red absorption test.
作者简介
A. Rusanov
Perspective Company
编辑信件的主要联系方式.
Email: alexander.l.rusanov@gmail.com
俄罗斯联邦, Novosibirsk
N. Luzgina
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: alexander.l.rusanov@gmail.com
俄罗斯联邦, Moscow
A. Lisitsa
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: alexander.l.rusanov@gmail.com
俄罗斯联邦, Moscow