Changing the Properties of Multipotent Mesenchymal Stromal Cells by IFNγ Administration
- 作者: Petinati N.1, Kapranov N.1, Bigil’deev A.1, Popova M.1, Davydova Y.1, Gal’tseva I.1, Drize N.1, Kuz’mina L.1, Parovichnikova E.1, Savchenko V.1
-
隶属关系:
- Hematology Research Center, Ministry of Health Care of the Russian Federation
- 期: 卷 163, 编号 2 (2017)
- 页面: 230-234
- 栏目: Microbiology and Immunology
- URL: https://journals.rcsi.science/0007-4888/article/view/238778
- DOI: https://doi.org/10.1007/s10517-017-3773-3
- ID: 238778
如何引用文章
详细
We studied changes in the population of human multipotent mesenchymal stromal cells activated by IFNγ. The cells were cultured under standard conditions; IFNγ was added in various concentrations for 4 h or over 2 passages. It was shown that the total cell production significantly decreased after long-term culturing with IFNγ, but 4-h exposure did not affect this parameter. After 4-h culturing, the expression levels of IDO1, CSF1, and IL-6 increased by 300, 7, and 2.4 times, respectively, and this increase persisted 1 and 2 days after removal of IFNγ from the culture medium. The expression of class I and II MHC (HLA) on cell surface practically did not change immediately after exposure to IFNγ, but during further culturing, HLA-ABC (MHC I) and HLA-DR (MHC II) expression significantly increased, which abolished the immune privilege in these cells, the property allowing clinical use of allogenic multipotent mesenchymal stromal cells. Multipotent mesenchymal stromal cells can suppress proliferation of lymphocytes. The degree of this suppression depends on individual properties of multipotent mesenchymal stromal cell donor. Treatment with IFNγ did not significantly affect the intensity of inhibition of lymphocyte proliferation by these cells.
作者简介
N. Petinati
Hematology Research Center, Ministry of Health Care of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
N. Kapranov
Hematology Research Center, Ministry of Health Care of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
A. Bigil’deev
Hematology Research Center, Ministry of Health Care of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
M. Popova
Hematology Research Center, Ministry of Health Care of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
Yu. Davydova
Hematology Research Center, Ministry of Health Care of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
I. Gal’tseva
Hematology Research Center, Ministry of Health Care of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
N. Drize
Hematology Research Center, Ministry of Health Care of the Russian Federation
编辑信件的主要联系方式.
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
L. Kuz’mina
Hematology Research Center, Ministry of Health Care of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
E. Parovichnikova
Hematology Research Center, Ministry of Health Care of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
V. Savchenko
Hematology Research Center, Ministry of Health Care of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow