Evaluation of Oxidative Metabolism in Leukocytes during Phagocytosis of Escherichia coli Carrying Genetic Constructs soxS::lux or katG::lux
- 作者: Karimov I.1, Deryabin D.2, Karimova D.1, Subbotina T.1, Manukhov I.3
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隶属关系:
- Orenburg State University
- State Research Center of Dermatology and Cosmetology, Ministry of Health of the Russian Federation
- State Research Institute of Genetics
- 期: 卷 161, 编号 2 (2016)
- 页面: 276-280
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237332
- DOI: https://doi.org/10.1007/s10517-016-3394-2
- ID: 237332
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详细
We studied ROS generation by human peripheral blood monocytes and granulocytes during phagocytosis of Escherichia coli soxS::lux or katG::lux responding by luminescence (bioluminescence) to the development of oxidative stress. Initially high sensitivity of the bioluminescent reaction of E. coli katG::lux strain to the effects of model ROS (KO2 and H2O2) and pronounced induction of luminescence upon contact with granulocytes, whereas E. coli soxS::lux demonstrated less pronounced reaction to chemical oxidants and bioluminescence was observed primarily upon contact with monocytes. A correlation was found between quantitative characteristics of E. coli katG::lux bioluminescence and luminol-dependent chemiluminescence of leukocytes in some patients, but no dependence of this kind was noted for E. coli soxS::lux. The results can provide experimental substantiation of a new approach for evaluation of ROS production by leukocytes during phagocytosis and choosing the optimal object for these studies.
作者简介
I. Karimov
Orenburg State University
Email: dgderyabin@yandex.ru
俄罗斯联邦, Orenburg
D. Deryabin
State Research Center of Dermatology and Cosmetology, Ministry of Health of the Russian Federation
编辑信件的主要联系方式.
Email: dgderyabin@yandex.ru
俄罗斯联邦, Moscow
D. Karimova
Orenburg State University
Email: dgderyabin@yandex.ru
俄罗斯联邦, Orenburg
T. Subbotina
Orenburg State University
Email: dgderyabin@yandex.ru
俄罗斯联邦, Orenburg
I. Manukhov
State Research Institute of Genetics
Email: dgderyabin@yandex.ru
俄罗斯联邦, Moscow