New Genetic Constructs for Generation of Stable Therapeutic Antibodies to Organophosphorus Toxins in Methylotrophic Yeasts Pichia Pastoris
- 作者: Mokrushina Y.1, Stepanova A.1, Bobik T.1, Smirnov I.1, Gabibov A.1
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隶属关系:
- Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- 期: 卷 161, 编号 1 (2016)
- 页面: 83-87
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237123
- DOI: https://doi.org/10.1007/s10517-016-3351-0
- ID: 237123
如何引用文章
详细
We propose a new method of obtaining of stable Fab-fragments of antibodies in Pichia pastoris expression system. Recently, we obtained Fab-fragments of antibodies neutralizing organophosphorus toxins. However, high yield of the target products was not attained because of high level of proteolytic degradation. In the present study, we identified sites of proteolytic degradation in Fab-fragments and endogenous proteases performing degradation, which allowed obtaining optimized genetic constructs for expression of antibody heavy chains (IgGγ1) and kappa and lambda isotypes of light chains. Co-transformation of these vectors allowed obtaining Fab-fragments of antibodies to organophosphorus toxins without proteolytic degradation of the product.
作者简介
Yu. Mokrushina
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: smirnov@mx.ibch.ru
俄罗斯联邦, Moscow
A. Stepanova
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: smirnov@mx.ibch.ru
俄罗斯联邦, Moscow
T. Bobik
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: smirnov@mx.ibch.ru
俄罗斯联邦, Moscow
I. Smirnov
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: smirnov@mx.ibch.ru
俄罗斯联邦, Moscow
A. Gabibov
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: smirnov@mx.ibch.ru
俄罗斯联邦, Moscow