Complex Approach to Xenobiotics Hepatotoxicity Testing using a Microfluidic System
- 作者: Alexandrova A.1, Pul’kova N.2, Sakharov D.1
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隶属关系:
- BioClinicum Research and Development Center
- Moscow State University of Mechanical Engineering (MAMI)
- 期: 卷 161, 编号 1 (2016)
- 页面: 50-53
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237082
- DOI: https://doi.org/10.1007/s10517-016-3342-1
- ID: 237082
如何引用文章
详细
We analyzed hepatotoxicity of three drugs: acetaminophen, metformin, and isoniazid. Spheroids of differentiated HepaRG cells cultured under microfluidic conditions were used as the model. Acute toxicity of substances was assessed by analyzing cell viability, while lactate concentration in the culture medium was used as the potential marker for evaluation of chronic exposure and non-lethal side effects of xenobiotics. The results were compared with mitochondrial activity and DNA fragmentation data. The efficiency and possibility of applying the integrated approach for assessment of drug hepatotoxicity are discussed.
作者简介
A. Alexandrova
BioClinicum Research and Development Center
编辑信件的主要联系方式.
Email: a.aleksandrova@bioclinicum.com
俄罗斯联邦, Moscow
N. Pul’kova
Moscow State University of Mechanical Engineering (MAMI)
Email: a.aleksandrova@bioclinicum.com
俄罗斯联邦, Moscow
D. Sakharov
BioClinicum Research and Development Center
Email: a.aleksandrova@bioclinicum.com
俄罗斯联邦, Moscow