Characteristics of Mel Ibr Melanoma Line Subclone after Treatment with Chicken Embryo Extract


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Аннотация

We continue analysis of the phenotype of human melanoma cell Mel Ibr subclone obtained previously by treatment of the parental cell line by chicken embryo extract. The present study is focused on detection of markers of epithelial-mesenchymal transition that determine enhanced metastatic and invasive potential of malignant tumors of various locations. Analysis of the expression of E-cadherin and vimentin genes in the subclone and parental cells detected activation of epithelial-mesenchymal transition in the subclone. Immunological markers CD90, CD271, and CD95 were present in the parental population, but were not detected on the subclone cells. In contrast to the parental line, cells of the analyzed subclone retain viability in serum-free medium and formed vessel-like structures characteristic of vasculogenic mimicry.

Об авторах

N. Suraeva

N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation

Автор, ответственный за переписку.
Email: nsuraeva@yandex.ru
Россия, Moscow

L. Morozova

N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation

Email: nsuraeva@yandex.ru
Россия, Moscow

O. Ryabaya

N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation

Email: nsuraeva@yandex.ru
Россия, Moscow

Yu. Khochenkova

N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation

Email: nsuraeva@yandex.ru
Россия, Moscow

A. Samoilov

All-Russian Research Institute of Preservation Technology

Email: nsuraeva@yandex.ru
Россия, Moscow Region

O. Burova

N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation

Email: nsuraeva@yandex.ru
Россия, Moscow

N. Golubtsova

N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation

Email: nsuraeva@yandex.ru
Россия, Moscow

A. Barmashov

N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation

Email: nsuraeva@yandex.ru
Россия, Moscow

M. Baryshnikova

N. N. Blokhin Russian Cancer Research Center, Ministry of Health of the Russian Federation

Email: nsuraeva@yandex.ru
Россия, Moscow


© Springer Science+Business Media, LLC, 2017

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