Production of Recombinant Polypeptides Containing One GA-Module and Analysis of Their Ability to Bind to Human Albumin
- Авторы: Bormotova E.1, Gupalova T.1
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Учреждения:
- Department of Molecular Microbiology Institute of Experimental Medicine
- Выпуск: Том 162, № 1 (2016)
- Страницы: 66-70
- Раздел: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237909
- DOI: https://doi.org/10.1007/s10517-016-3547-3
- ID: 237909
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Аннотация
Surface proteins of many bacterial species interact with human serum albumin (HSA) via a special region of amino acid sequence termed GA module. For instance, surface peptostreptococcal albumin-binding protein of anaerobic bacteria Peptostreptococcus magnus contains one HSA-binding GA-module. Protein G from group G and C Streptococcus strains isolated from humans has HSA-binding region consisting of three GA-modules. HSA-binding protein containing two GA-modules was found in strains of group G Streptococcus of animal origin. We obtained two recombinant polypeptides GA1 and GA2 congaing one GA-module each. Recombinant polypeptide with two GA-modules binds HSA with a much higher affinity than polypeptides GA1 and GA2 containing one GA-module. Polypeptide with the second GAmodule more effectively binds HSA than polypeptides with the GA-module.
Об авторах
E. Bormotova
Department of Molecular Microbiology Institute of Experimental Medicine
Email: tvgupalova@rambler.ru
Россия, St. Petersburg
T. Gupalova
Department of Molecular Microbiology Institute of Experimental Medicine
Автор, ответственный за переписку.
Email: tvgupalova@rambler.ru
Россия, St. Petersburg
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